Proteasome inhibitors (e.g., bortezomib, MG132) are known to enhance adeno-associated virus (AAV) transduction; however, whether this results from pleotropic proteasome inhibition or off-target serine and/or cysteine protease inhibition remains unresolved. Here, we examined recombinant AAV (rAAV) effects of a new proteasome inhibitor, carfilzomib, which specifically inhibits chymotrypsin-like p...

Citation: Ubuka T, Moriya S, Soga T and Parhar I (2018) Identification of Transmembrane Protease Serine 2 and Forkhead Box A1 As the Potential Bisphenol A Responsive Genes in the Neonatal Male Rat Brain. Front. Endocrinol. 9:139. doi: 10.3389/fendo.2018.00139 identification of Transmembrane Protease serine 2 and Forkhead box a1 as the Potential bisphenol a responsive genes in the neonatal Male ...

Serine proteases are widely distributed in viperid snake venoms, but rare in elapid snake venoms. Previously, we have identified a fibrinogenolytic enzyme termed OhS1 from the venom of Ophiophagus hannah. The results indicated that OhS1 might be a serine protease, but there was no structural evidence previously. In the present study, the primary structure of OhS1 was determined by protein seque...

The baker's yeast, Saccharomyces cerevisiae, is also capable of undergoing programmed cell death or apoptosis, for example in response to viral infection as well as during chronological and replicative aging. Intrinsically, programmed cell death in yeast can be induced by, for example, H2O2, acetic acid or the mating-type pheromone. A number of evolutionarily conserved apoptosis-regulatory prot...

The serine protease urokinase-type plasminogen activator (uPA) and its inhibitor, PAI-1, are key players in a proteolytic cascade involved in physiological and pathophysiological degradation and remodelling of the extracellular matrix. uPA, when bound to its cellular receptor uPA-R (CD87) efficiently converts plasminogen into the broad spectrum serine protease plasmin; its action on plasminogen...

In recent decades, several canonical serine protease inhibitor families have been classified and characterized. In contrast to most trypsin inhibitors, those from garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) do not share sequence similarity and have been proposed to form the new Mirabilis serine protease inhibitor family. These 30-40-amino-acid inhibitors possess a def...

The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions in the cleft, with the known sequences at the cleavage site of type I collagen...

Diisopropylfiuorophosphate {OFP), a general serine protease inhibitor, inhibited the DNA fragme"tatioll and cell death in MDCK cells treated with ricin, modeccin, Ilseudomonas toxin, or dipbtberia toxin. Atrypsin-like serine protease inhibitor, IVLtosyl・L-lysine chleromethyl ketone (TLCK) also prevented ricin-induced DNA fragmentation and cel] death, albeit less effectiyely than DFP. Microscopi...

TMPRSS2 is a type II transmembrane-bound serine protease that has gained interest owing to its highly localized expression in the prostate and its overexpression in neoplastic prostate epithelium. Once activated, the serine protease domain of TMPRSS2 is released from the cell surface into the extracellular space. PAR (protease-activated receptor)-2 belongs to a family of G-protein-coupled recep...

The yeast-like fungus Aureobasidium pullulans PL5 is a microbial antagonist against postharvest pathogens of fruits. The strain is able to produce hydrolases, including glucanases, chitinases and proteases. The alkaline serine protease gene ALP5 from A. pullulans was cloned, inserted into the vector pPIC9 to construct pPIC9/ALP5, and then expressed in Pichia pastoris strain KM71. ALP5 had a mol...