In order to study on some of pistachio cultivars’ contamination of Khorasan-e-Razavi province,eight cultivars of pistachio kernels were collected from the pistachio production areas of this province. Using serial dilution method, 10 grams of ground pistachio was moved to 90 ml water peptone and continuous dilutions 10-1, 10-2 were prepared. Then 0.1 ml from each dilution was inoculated in three...

Pseudomonas oleovorans NCIMB 6576 and Ralstonia eutropha 10442 were used for the production of Polyhydroxyalkanoates (PHA) from industrial waste cooking oils, bacteria cultured on tryptone soya broth (TSB) Tryptone agar (TSA). The growth pattern bacteria, serial dilution viable counting was done using Miles Misra method, 0.5ml (500 µl) sample transferred aseptically into test tubes filled with ...

Purpose: A deficit was identified in the traditional method of making homeopathic dilutions by 1:9 or 1:99 serial based on physical units mass volume. When chemical unit mole is considered, mass-based dilution nitroglycerin ethyl alcohol has a 1:48.8 molar ratio. In order to provide ratio, ratios required for formulations with lithium carbonate: lactose (diluent) and lead phosphate: are 1:41.7 ...

A simplified method has been developed for agar dilution antimicrobial susceptibility testing of anaerobic bacteria, designed to economize on time and money when only a few isolates need to be tested. The procedure is based on the principle of using filter paper disks as carriers of the antibiotic and 35- by 10-mm petri dishes which, when inoculated with the Steers replicator, can test up to fo...

The present study was carried out to evaluate the in vitro antimicrobial and antioxidant activity of Emblica officinalis extract powder. assessed against gram positive negative bacteria namely E.coli, Pseudomonas fluoresces, Bacillus subtilis, S.aureus, by agar well diffusion method. Quantitative susceptibility testing method, i.e., determination minimum inhibitory concentration (MIC) serial br...

background & aim: the major aim of this study was to determine the antibacterial activity of the essential oils from five medicinal plants. experimental: the hydro-distillation essential oils from five plant species, including satureja bachtiarica, zataria multiflora, thymus daenensis, thymus carmanicus, and ziziphora tenuior (lamiaceae) which are endemic in iran. in-vitro antibacterial tes...

background drug resistance of microorganisms to antimicrobial agents is on the rise. therefore, an alternative route to overcome drug resistance of various microorganisms is needed. objectives this study was conducted to assess antibacterial activity of silver nanoparticles (agnps) produced by satureja hortensis extract against bacillus cereus isolated from soil of sistan plain (zabol, southeas...

Variable-number tandem-repeat (VNTR) typing with a panel of 24 loci is the current gold standard in the molecular typing of Mycobacterium tuberculosis complex isolates. However, because of technical problems, a part of the loci often cannot be amplified by multiplex PCRs. Therefore, a considerable number of single-locus PCRs have to be performed for the loci with missing results, which impairs ...

A multinational interlaboratory task force explored the important variables of platelet reference counting and developed a candidate flow cytometric reference method based on the RBC/platelet ratio. A multicenter comparison was performed to determine whether the method met the necessary criteria and was precise enough to be recommended as a new reference method. Each laboratory analyzed serial ...