نتایج جستجو برای: sensitive gel electrophoresis csge

تعداد نتایج: 395836  

Journal: :BioTechniques 2000
E P Rogakou C Redon C Boon K Johnson W M Bonner

with 5% acetic acid and, after 10 min, the gel was finally washed in distilled water and air-dried onto the glass support. Air-drying allows the long and thin gel to be easily submitted to photographic and/or digital recording using a sequencing gel-sized scanner, such as the HP ScanJet 4C/T (Hewlett-Packard, Palo Alto, CA, USA). This avoids wethandling or the need of an apparatus for gel-dryin...

Journal: :Journal of medical genetics 2002
D J Halliday S Hutchinson L Lonie J A Hurst H Firth P A Handford P Wordsworth

Marfan syndrome (MFS) is one of the major heritable disorders of connective tissue with a prevalence of between 1 in 5-10 000. 2 It is characterised by features in the cardiovascular, ocular, and musculoskeletal systems and the Ghent criteria form a useful framework for its diagnosis. Mutations in FBN1 encoding the extracellular matrix protein fibrillin-1 classically cause MFS. Fibrillin-1, com...

Journal: :Cancer research 1977
G L Wright P F Schellhammer R L Faulconer

of human tumors (1-10, 12-16). Since these extracts un doubtedly contain a spectrum of antigens, normal as well as tumor related, the isolation and purification of the TAA's would facilitate greatly the development of sensitive and specific immunological assays for the diagnosis and man agement of the cancer patient. Attempts to isolate the TAA's with the use of a variety of fractionation proce...

Journal: :caspian journal of environmental sciences 2007
m. mondal

the domesticated silkworm, bombyx mori linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. the silkworm, b. mori produces massive amount of silk proteins during the final stage of larval development. these proteins are stored in the middle silk gland and they are dischar...

Journal: :iranian red crescent medical journal 0
aziz japoni alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran mojtaba anvarinejad alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran; alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran. tel: +98-7116474264, fax: +98-7116474303 shohreh farshad alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran giovanni m giammanco department of health promotion sciences, university of palermo, palermo, italy noroddin rafaatpour alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran ebrahim alipour alborzi clinical microbiology research center, nemazee teaching hospital, shiraz university of medical sciences, shiraz, ir iran

background: failure in the treatment of burn patients infected with pseudomonas aeruginosa could happen as a result of the acquisition of antibiotic resistance, including carbapenems. objectives: the aim of the present study was to investigate the phenotypic and genotypic characteristics of the pseudomonas aeruginosa strains, isolated from burn patients. patients and methods: during a 12 month ...

Journal: :journal of agricultural science and technology 0
j. kutasi biofil ltd., budapest, hungary. r. kovacs biofil ltd., budapest, hungary. i. puspan biofil ltd., budapest, hungary. j. makk department of microbiology, eötvös loránd university, budapest, hungary. k. takacs fermentia ltd., budapest, hungary. b. erdelyi fermentia ltd., budapest, hungary. cs. imre

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

Journal: :avicenna journal of medical biotechnology 0

stress is a threatening factor that all living organisms encounter throughout life. depending on the type of stress, there are several mechanisms for keeping body homeostasis to minimize stress effects. brain is an organ which shows high sensitivity to stress conditions. although many studies have shown induced-stress effects on rat embryos, little is known about the mechanisms involved in copi...

Journal: :iranian journal of microbiology 0
parviz mohajeri department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, iran. abbas farahani student research committee, kermanshah university of medical sciences, kermanshah, iran. mohammad-mehdi feizabadi department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran. hosnieh ketabi student research committee, kermanshah university of medical sciences, kermanshah, iran. ramin abiri department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, iran. farid najafi kermanshah health research center (khrc), school of health, kermanshah university of medical sciences, kermanshah, iran.

background and objective: acinetobacter baumannii is an aerobic non-motile gram-negative bacterial pathogen that is resistant to most antibiotics. carbapenems are the most common antibiotics for the treatment of infections caused by this pathogen. mechanisms of antibiotic-resistance in a. baumannii are mainly mediated by efflux pumps-lactamases. the aim of this study was to determine antibiotic...

Journal: :iranian journal of microbiology 0
arash ghodousi department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran. s vatani adept. of microbiology, school of medicine & infectious and tropical diseases research centre, ahvaz jundishapur university of medical sciences, ahvaz, iran.. davood darban-sarokhalil department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran. maryam omrani department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran. aa fooladi research center of molecular biology, department of pathology, school of medical sciences, baqiyatallah university of medical sciences, tehran, iran. ad khosaravi adept. of microbiology, school of medicine & infectious and tropical diseases research centre, ahvaz jundishapur university of medical sciences, ahvaz, iran.

a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

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