Although exposure of Trichomonas vaginalis to human semen is of short duration, any effect that this fluid may have on the urogenital protozoon could affect its transmission, especially if only few trichomonads are present. Small numbers of parasites (about 2500/ml semen) incubated in semen from different donors at 37 degrees C, were found to survive or grow for up to 12 hours in all samples an...

The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...

Considering the venereal transmission of visceral leishmaniasis from dogs to bitches, the aim of this study was to verify if the penile surface and smegma from infected dogs can be the source of parasites in bitches. Twelve Leishmania infantum infected dogs had semen and smegma samples collected for submission to PCR identification of the DNA of the parasite. Semen (41.7%) and smegma (50.0%) ha...

A field trial was conducted on 36 farms in the Netherlands to compare the fertilizing capacity of fresh and frozen-thawed boar spermatozoa. Four-hundred and fifty-one sows were artificially inseminated with semen that had been frozen and thawed according to the Beltsville Method or diluted in Kiev extender and inseminated on the day of collection. Twelve boars of the Dutch Landrace and Dutch La...

There are currently no WHO guidelines on the indications for semen culture; however, semen cultures are performed in the evaluation of male infertility and the assisted reproductive technology (ART) process. The relevance and significance of positive semen cultures is widely debated in the literature, with no current consensus on the usefulness of this test in relation to male infertility. We r...

The assessment of spermatozoa motility is one of the most often used parameters for semen evaluation. The study was conducted at Poultry Research Station, Madhavaram Milk Colony, Chennai600 051, to  nd the effect of different semen diluents and dilution ratio on per cent spermatozoa motility of pooled guinea fowl semen at 5o C under short term storage(0, 3 and 6 Hours). Irrespective of the sto...

Dairy farmers have been able to use sexed semen from dairy breeds to pre-determine the sex of calves since 2000, sexed semen from beef bulls is not currently commercially available, but is expected within 2 years. A survey of a stratified random sample of suckler cow farmers is used to identify the potential uptake of sexed semen when it becomes available using a logistic limited dependent vari...

Determining the initial quality of a boar ejaculate is the first step in semen processing and should ensure that prior to further processing, a high quality artificial insemination dose of semen will be produced. Effective screening methods for ejaculates prior to processing are necessary for improving on farm reproductive performances. Ideally, ejaculates that are thoroughly evaluated prior to...

Objective – To study the incidence of male factor infertility and type of semen abnormality in the farmer couple attending our infertility outpatient clinic. MethodDatabase of 3100 patients with male factor infertility were retrospectively reviewed. Patients were divided in two groups for analysis: group 1 – farmer , group 2 – non farmer. Records of semen analysis and serum FSH (Follicle stimul...

Epidemiological data indicate a sexual route of transmission of acquired immune deficiency syndrome (AIDS) associated Kaposi's sarcoma. Recently human herpes virus 8 (HHV-8) has been proposed as the aetiological agent for development of Kaposi's sarcoma. Further the virus has been reported in semen obtained from healthy men. In Denmark strict biochemical and microbiological criteria are used in...