Little is known about how cultivation legacies affect the outcome of rehabilitation seedings in the Great Basin, even though both frequently co-occur on the same lands. Similarly, there is little known about how these legacies affect native species reestablishment into these seedings. We examined these legacy effects by comparing areas historically cultivated and seeded to adjacent areas that w...

Mouse mammary tumors were dissociated to single cells. These were seeded in high-serum medium at three different densities. Three different culture morphologies and three different saturation densities of cells resulted. In contrast, embryo fibroblasts cultured under the same conditions grew to the same end point over a wide range of seeding densities. Growth of these neoplastic cells is densit...

Fecal seeding by livestock may be an effective, low-cost means of rangeland restoration. We compared recruitment of switchgrass (Panicum virgatum L.) from seed fed to cattle and deposited in dung to that of broadcast-seeded plots receiving a comparable number of unfed seed. Although germinability of seed passed through livestock (52 to 62%) was reduced relative to that of broadcast seed (85 to ...

Cellular fibronectin is deposited on tissue culture polystyrene during the adhesion and spreading of cultured human endothelial cells (HEC). Following the seeding of HEC upon this polymer, larger amounts of fibronectin are deposited as both cell density and incubation time increase. Our results indicate that the ability to deposit cellular fibronectin onto a polymeric surface is a condition for...

BACKGROUND AND AIM OF THE STUDY A new-generation heart valve was developed to improve long-term durability. The study aim was to evaluate the need for in-vitro autologous endothelial cell seeding of a decellularized valve scaffold. METHODS A seeded (group I, n = 6) or non-seeded (group II, n=6) decellularized valve was implanted into the right ventricular outflow tract of 12 juvenile sheep. I...

Effective cell seeding throughout the tissue scaffold often determines the success of tissue-engineering products, although most current methods focus on determining the total number, not the distribution, of the cells associated with tissue-engineering constructs. The purpose of this investigation was to establish a quick, convenient, and efficient method to quantify cell survival, distributio...

Rice in the U.S. is frequently seeded at low rates and treated before sowing with neonicotinoid or anthranilic diamide insecticides to target the rice water weevil. A previous study of the influence of seeding rate on rice water weevil densities showed an inverse relationship between seeding rates and immature weevil densities. This study investigated interactive effects of seeding rate and see...

BACKGROUND Tissue engineering with cell seeded biodegradable material has attracted attention as a novel means of treating the severely impaired heart. Here, we consider optimal preparation of a durable biograft using dynamic and static cultures. METHODS Vascular smooth muscle cells (VSMCs) derived from the rat aorta were seeded onto biodegradable material P (LA/CL) (poly-L-lactide-ε-caprolac...

BACKGROUND The anterior chamber has been shown by pharmacokinetic studies to represent a sanctuary never achieving a tumoricidal dose with the present administration routes, such as systemic, intra-arterial, or intravitreal injections. METHOD A novel intracameral chemotherapy technique is described to control aqueous seeding in a pilot unilateral group E retinoblastoma case with primary aqueo...