Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw ML populations created from crosses between Soya × Kalubungula, Sowor...

The ER (endoplasmic reticulum) has long been considered the plant cell compartment within which protein disulfide bond formation occurs. Members of the ER-located PDI (protein disulfide isomerase) family are responsible for oxidizing, reducing and isomerizing disulfide bonds, as well as functioning as chaperones to newly synthesized proteins. In the present study we demonstrate that an abundant...

To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, determined by combustion analysis of nitrogen, revealed a decline in the protein content after decades...

Plant vacuoles have multiple functions: they can act both as digestive organelles and as receptacles for storage proteins. Different types of vacuoles can coexist in the same cell, which adds complexity to the process of targeting to these compartments. A fuller understanding of this process is of evident value when endeavouring to exploit the plant secretory pathway for heterologous protein pr...

One hundred and fifty one accessions of groundnut representing five continents were evaluated for total seed protein by SDS-PAGE using slab type gel electrophoresis with 11.25 % polyacrylamide gel. Five major bands were recorded and most of the accessions were similar and only 8 differed for one band. Due to low genetic diversity for SDS-PAGE, 2D-electrophoresis was suggested to separate variou...

School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India Biological Science Group, Birla Institute of Technology, Pilani, 333 031, India Biotechnology Laboratory, Indian Institute of Vegetable Research, P O Jakhini-Shahanshahpur, Varanasi 221 305, India Laboratory of Plant Molecular Morphogenesis, Faculty of Biological Sciences, Nara Institute of Science an...

Secale montanum is one of the important perennial grasses growingnaturally in arid to semiarid pastures and rangelands, with a typical Mediterraneanclimate, in northern and western Iran at altitudes of 800-2900 m. In this paper, seedstorage protein profiles of nine wild populations of S. montanum from differentregions of Iran and their phenotypically superior progenies as well as a multi-origin...