The study was aimed at assessing the genetic variation and relatedness among four Capsicum varieties through electrophoretic separation of their leaf and seed proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total seed and leaf proteins were extracted and separated on 12% polyacrylamide gels using standard protocols. Distinct polymorphism in electrophoretic ban...

The intrinsic membrane proteins of the endoplasmic reticulum from endosperm of maturing and germinated seedlings of castor bean (Ricinus communis) were studied. Preparations were simultaneously subjected to two-dimensional polyacrylamide gel electrophoresis. At least 30 separate proteins were distinguished by staining the gels with Coomassie R-250. The characteristic protein profiles obtained f...

We have measured the numbers of genes coding for the three seed storage proteins, vicilin, convicilin and legumin, in a number of Pisum genotypes of variant protein composition. No difference in gene number existed among P. sativum genotypes for any of the proteins. There were differences in the number of genes coding for individual proteins with approximately 11 genes (per haploid genome) for ...

Several classes of seed proteins limit the utilisation of plant proteins in human and farm animal diets, while plant foods have much to offer to the sustainable intensification of food/feed production and to human health. Reduction or removal of these proteins could greatly enhance seed protein quality and various strategies have been used to try to achieve this with limited success. We investi...

The success of seed germination and establishment of a normal seedling are key determinants of plant species propagation. At present, only a few studies have focused on the genetic control of seed germination by using a proteomic approach in Brassica napus. In the present study, the protein expression pattern of seed germination was investigated using differential fluorescence two-dimensional g...

The seed coat serves as a multifunctional organ with a role in protection and for the supply of nutrients to the embryo sac during development. The composition of the legume seed coat differs from other seed tissues in many ways including its protein composition. An abundant 24 kDa protein (SC24) has been purified and identified from soybean (Glycine max [L.] Merr) seed hulls. The corresponding...

electrophoresis of proteins, especially seed proteins, and karyological studies are useful techniques for determining species and solving the problems of modern taxonomy. the present study was carried out on two species of the apocynaceae family, rhazya stricta decasine and nerium 01eancder.l. seed samples of these two genera were taken from the south-east of iran and sds-page analysis of their...

Seed-type vacuolar processing enzyme (VPE) activity is predicted to be essential for post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds. To test this hypothesis, we examined the protein profiles of developing and germinating seeds from Arabidopsis plants containing transposon-insertional knockout mutations in the genes that encode the two ...

The composition of seed storage proteins (SSPs) in olive endosperm and cotyledon has been analyzed. Precursor forms of these proteins are made up of individual proteins, which have been purified to homogeneity and further named p1-p5 (20.5, 21.5, 25.5, 27.5, and 30 kDa, respectively). N-terminal sequences of p1 and p2 proteins displayed relevant homology to the basic subunit of the 11S family o...

Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for...