نتایج جستجو برای: scnt embryo

تعداد نتایج: 67711  

Journal: :Methods in cell biology 2011
Kannika Siripattarapravat Jose B Cibelli

Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish research community. In this chapter, we met...

2011
Rangsun Parnpai Kanokwan Srirattana Sumeth Imsoonthornruksa Mariena Ketudat-Cairns

It has now been 15 years since the first cloned sheep was born using somatic cell nuclear transfer (SCNT). This technique provides a unique tool for preservation of valuable individuals, livestock propagation, genetically modified animals, production research of biomedicine and conservation of endangered species. In this review, research of the SCNT in livestock and endangered species, includin...

2017
Dong-Hyuk Shin Jeoung-Eun Lee Jin Hee Eum Young Gie Chung Hoon Taek Lee Dong Ryul Lee

Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and...

Journal: :Gerontology 2017
Jörg Patrick Burgstaller Gottfried Brem

The number of species for which somatic cell nuclear transfer (SCNT) protocols are established is still increasing. Due to the high number of cloned farm, companion, and sport animals, the topic of animal cloning never ceases to be of public interest. Numerous studies cover the health status of SCNT-derived animals, but very few cover the effects of SCNT on aging. However, only cloned animals t...

2014
Sang Kyu Park Sangho Roh Jong-Im Park

Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated ...

Journal: :Ciencia Rural 2021

ABSTRACT: This study evaluated the viability of Nellore cloned calves derived from somatic cell nuclear transfer (SCNT) and compare their with animals same breed in vitro fertilization (IVF). Thus, two groups were formed. Group I (GI) consisted 10 SCNT group II (GII) IVF. The differences detected between physical examination respiratory tract GI, which represented most common clinical-pathologi...

2016
Byungkuk Min Sunwha Cho Jung Sun Park Kyuheum Jeon Yong-Kook Kang

Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We obse...

Forouzanfar M Gourabi H Hajian M Hosseini M Jafarpour F Ostadhosseini S Shahverdi AH

Background: At the outset of the somatic cell nuclear transfer (SCNT) process, the chromatin structure of the somatic cell which governs its state of differentiation undergoes dramatic changes, called reprogramming, and is compelled back to the embryonic stage. However, the overall epigenetic makeup of the resultant cloned embryos has been acknowledged far different from the fertilized embryos....

2014
Ton Yoisungnern Ashit Kumar Paul

A number of animals with genetically identical appearance can be produced by somatic cell nuclear transfer (SCNT). From current advancement of SCNT and molecular techniques, production of a transgenic animal becomes easier. Although cloning efficiency in goat is low, the ability to propagate genetically identical animals, with a gene or genes of interest, would be important for increasing produ...

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