A computational procedure is described for assigning the absolute hand of the structure of a protein or assembly determined by single-particle electron microscopy. The procedure requires a pair of micrographs of the same particle field recorded at two tilt angles of a single tilt-axis specimen holder together with the three-dimensional map whose hand is being determined. For orientations determ...

This article presents the complementary data regarding compatibilization of chitosan/κ-carrageenan polyelectrolyte complex for synthesizing of a soft film using ascorbic acid. It includes the thermal-theory for estimating the degradation of κ-carrageenan, as reflected in alteration of the structural properties of the blend. The data has been provided to demonstrate that the blend solution based...

In a paper in the preceding issue of this Journal Morgan, Howe, Rose, and Moore (1) have described their studies of electron micrographs of sections of tissue infected with RI-APC viruses. The virus particle aggregates appear most frequently as well ordered two-dimensional arrays. Studies of serial sections have shown that these two-dimensional nets are planes through three-dimensional virus cr...

lta zeolite nanoparticles was successfully synthesized from clear solution under controlled conditions using hydrothermal method using tetramethyl ammonium hydroxide as a structural directing agent. the as-synthesized lta zeolite particles were characterized by powder x-ray diffraction (xrd) analysis, scanning electron microscopy (sem), fourier transform infrared (ft-ir) spectroscopy, energy di...

nanosized metal oxides dispersed polymer composites constitute a fascinating class of polymer composite materials. synthesis of such composite materials through solvent casting enhances the polymer synthetic technology. solvent casting method was used to prepare cobalt oxide (co3o4) dispersed poly (methyl methacrylate) (pmma) nano composite. x-ray diffraction tool is used to know the structural...

Single particle analysis is a valuable tool in cryo-electron microscopy for determining the structure of biological complexes. However, the conformational state and the preparation of the sample are factors that play a critical role in the ultimate attainable resolution. In some cases extensive analysis at the microscope of a sample under different conditions is required to derive the optimal a...

AIMS To characterise a strain of Gram negative aerobic straight or slightly curved rods (HKU3) isolated from the blood culture of a 9 year old Chinese boy with neutropenic fever and pseudobacteraemia. METHODS The isolate was phenotypically investigated by standard biochemical methods using conventional biochemical tests, scanning electron microscopy, and transmission electron microscopy. Geno...

  The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...

A detailed study of stereoscopy of tissue sections with the transmission electron microscope has been carried out using different tilt angles and staining techniques, aided with models. Pictures with tilt angle difference of 20 or more can be fused to give pairs of micrographs showing stereodepth. Cytoplasmic filaments, glycogen and ribosomal granules give good stereodepth. Oblique membranes, e...