We hypothesize that we can exploit what Nature has already evolved by manipulating the alpha-helix molecular recognition scaffold. Therefore, minimalist proteins capable of sequence-specific, high-affinity binding of DNA were generated to probe how proteins are used and can be used to recognize DNA. The already minimal basic region/leucine zipper motif (bZIP) of GCN4 was reduced to an even more...

Abstract This study aims to produce composite nanofibers from polylactic acid (PLA) and silk fibroin (SF) with mechanical properties similar that of a native meniscus. SF is natural protein, well known for its good biocompatibility biodegradability but it has poor compared synthetic polymers. contains proteins which can promote cell adhesion proliferation required tissue engineering scaffold. P...

Regulatory complexes formed on mRNAs control translation, stability, and localization. These complexes possess two activities: one that binds RNA and another--the effector--that elicits a biological function. The Pumilio and FBF (PUF) protein family of RNA binding proteins provides a versatile scaffold to design and select proteins with new specificities. Here, the PUF scaffold is used to targe...