Diagnosis of Toxoplasma gondii infection is difficult, especially in immunosuppressed people. The AIDS epidemic has increased the number of people at risk and increased the need for better diagnostic methods. We have compared three methods for detection of T. gondii parasitemia. Rabbits were infected subcutaneously with 10(4) T. gondii tachyzoites. Blood samples were obtained, and buffy coat or...

infection by toxoplasma gondii is widespread inhumans and many other species of warm-blooded animals. it can cause significant morbidity and mortality in the developing fetus and in immunocompromised individuals,including humans with acquired immunodeficiency syndrome (aids) or submitted to cancer chemotherapy.among livestock, sheep and goat are more widely infected with t. gondii. this parasit...

The t(12;21) (p13;q22) translocation resulting in ETV6/RUNX1 (previously named TEL/AML1) gene fusion is present in about 25% of children with precursor B-lineage acute lymphoblastic leukemia (B-ALL). We successfully tested 275 precursor B-ALL samples from children aged 1 to 17 years to determine the relation between t(12;21) and in vitro cellular drug resistance, measured by the fluorometric mi...

BACKGROUND PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its complexity and the lack of standardization. METHODOLOGY We here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR ...

In an attempt to assess the possible risk to humans of soil-transmitted canine helminths of of zoonotic significance, 161 faecal samples and 81 soil samples were collected from 6 public parks and playgrounds in Harare between March and June 1998 and examined for nematode ova. Of the 161 faecal samples collected, 17.4% were positive for Ancylostoma sp. ova and 5.6% were positive for T. canis ova...

Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxoplasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by neste...

In the electrically correlated metal URhGe ferromagnetic order (T C = 9.5 K) and superconductivity (T s = 0.25 K) coexist at ambient pressure. Here we report on chemical substitution of Rh by Ru, which enables one to tune the Curie temperature to T = 0. URuGe has a paramagnetic ground state and is isostructural to URhGe. We have prepared a series of polycrystalline URh 1−x Ru x Ge samples over ...

Background: The diagnosis of T-cell non-Hodgkin lymphomas (NHL) is challenging. Many cases T-NHL have normal immunophenotypes, and currently available clonality assays limitations, including suboptimal sensitivity. development a monoclonal antibody (mAb) specific for receptor β constant region 1 (TRBC1) provides an alternative to discriminate clonal T cells. aim this study was evaluate the diag...