A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...

Salmonella enterica enterica Salmonella ., Salmonella . S. typhi

  A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

Among foodborne pathogens, Salmonella present a significant public health hazard as Salmonella caused the greatest number of foodborne deaths in the United States and was among the top three most frequent causes of bacterial foodborne illness in the United States (Mead et al., 1999). In 2002, Salmonella Typhimurium and Salmonella Newport accounted for 2 of the top 3 most common Salmonella serov...

To develop a live vaccine strain against fowl typhoid and paratyphoid caused by Salmonella serovar Gallinarum biovar Gallinarum (Salmonella Gallinarum) and Salmonella serovar Enteritidis (Salmonella Enteritidis), respectively, several nalidixic acid resistant mutants were selected from lipopolysaccharide (LPS) rough strains of Salmonella Gallinarum that escaped from fatal infection of a LPS-bin...

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of t...

The objectives of this study were to enumerate Salmonella enterica serovar Enteritidis colonization in fecal, cecal, and internal organs, and to compare the level of virulence gene expression (hilA) of experimentally challenged laying hens fed different dietary molt-induction regimens. Twelve Salmonella-free Single Comb Leghorn hens (>50 wk old) hens were randomly assigned to each of 6 treatmen...

Because challenge models to infect peripheral lymph nodes (PLNs) with Salmonella have not been reported, we performed a series of experiments to develop and refine challenge models to evaluate an intervention applied at the animal level and to provide initial estimates of efficacy of an intervention (i.e., a vaccine) to aid in the design of future studies. In each of four experiments, steers (c...

Salmonellosis is a very important disease of avian species because of its huge economic impact, worldwide distribution and difficulty posed in its control. Fowl typhoid and pullorum disease, is caused by Salmonella enterica subsp enterica serovar Gallinarum biovar Gallinarum and Pullorum. The purpose of this study was to investigate the biochemical characteristics and antimicrobial susceptibili...

Introduction: Zataria multiflora is a plant of the Lamias family. The most effective antimicrobial composition is its thymol and carvacrol. Salmonella typhimurium is a genus of Salmonella from the Enterobacteriaceae family. This gram-negative basil, optional aerobic or anaerobic, cause diseases such as inflammation of the intestine, systemic infection, and intestinal fever. Th...