Treatment failure occurs in up to 30% of patients treated with steroids for inflammatory diseases. The aim of this study was to explore the potential role of 21 cytokines in steroid-resistant inflammatory disease and to develop methods to restore steroid sensitivity through cytokine manipulation. The dexamethasone inhibition of lymphocyte proliferation assay correlates with the outcome of stero...

Phytochemical and antibacterial activity assay of methanol extract from Suji leaves (Pleomele angustifolia) were carried out. The aims this study are to determine secondary metabolites the against Streptococcus sobrinus KCCM 11898 mutans ATCC 25175 by using agar well diffusion. results showed that suji contain phenolic, triterpenoid steroid constituents. With 2%, displayed with diameter inhibit...

The aim of this study was to compare antioxidant levels of saliva in patients with cirrhosis and liver transplantation. This study was carried out on 45 subjects referred to the Gastroenterohepatology Clinic of Dicle University Medical Faculty. These subjects were composed of cirrhosis, transplanted patients, and control group with no liver problems. The saliva samples were taken in the morning...

Three laboratories compared their routine steroid binding assays with the Abbott estrogen receptor-enzyme immunoassay (ER-EIA) to determine ER in breast cancer cytosols. Each laboratory was first trained to use the ER-EIA kit and then performed routine proficiency panels to determine assay reproducibility. The frozen panels prepared from MCF-7 cytosol produced good intraassay results but the be...

When quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. The Invader assay is a convenient method because it does not involve gene amplification. The purpose of this study was to evaluate the validity of the Invader assay to quantify periodontopathic bacteria. The Invader technology was applied in quantifying five pe...

Introduction: Correlation between circulating sex steroid levels and breast cancer has been controversial, with measurement of free, or bioavailable hormone rarely available. Salivary hormone levels represent the bioavailable fraction. We aimed to assess correlation between salivary sex steroid levels and breast cancer prevalence. Methods: Salivary hormone levels of testosterone (T), Estradiol ...

BACKGROUND Detection limit challenges associated with measuring low-abundance protein biomarkers can be addressed with hybrid immunoaffinity-mass spectrometric assays, such as antipeptide antibody capture followed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Popular assay formats use magnetic bead-based immunoaffinity enrichment and nanoflow LC-MS/MS or high-flow immunoaffinity...

OBJECTIVE To compare serum, salivary and fecal IgA responses in infants and adults following rotavirus vaccination. STUDY DESIGN Laboratory testing of samples from clinical trials. SETTING Medical College Hospital. PARTICIPANTS 13 healthy adult volunteers not given vaccine, 20 healthy adult volunteers given one dose of bovine rotavirus tetravalent vaccine (Shantha Biotechnics), and 88 inf...

BACKGROUND Self-report measures indicate that Yoga practices are perceived to reduce stress; however, molecular mechanisms through which YB affects stress are just beginning to be understood. While invasive sampling such as blood has been widely used to measure biological indicators such as pro-inflammatory biomarkers, the use of saliva to measure changes in various biomolecules has been increa...

BACKGROUND Steroid immunoassays originally required solvent extraction, chromatography, and structurally authentic tracers to avoid interference from steroid cross-reactivity and matrix effects. The demand for steroid assays has driven assay simplification, bypassing this triplet of validity criteria to allow use of unextracted serum, which has introduced bias and nonspecificity at low steroid ...