Rifampin is the most potent drug used in the treatment of disease due to Mycobacterium kansasii. A 69-bp fragment of rpoB, the gene that encodes the beta subunit of the bacterial RNA polymerase, was sequenced and found to be identical in five rifampin-susceptible clinical isolates of M. kansasii. This sequence showed 87% homology with the Mycobacterium tuberculosis gene, with an identical deduc...

OBJECTIVES to investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB. METHODS a total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes en...

the upstream rpoBF1 but with a DNA uptake sequence (in lower case) that was added at the 5′ end to permit DNA transformation (7). The transformant strain NM05-08 was resistant to rifampin (MIC >32 mg/L), and the sequence of the rpoB gene confirmed the His→Tyr mutation. When compared to the parental isolate (LNP21362), strain NM05-08 showed reduced virulence. Indeed, bacterial loads were similar...

Spirochetes are emerging pathogens for which culture and identification are partly unresolved. In fact, 16S rRNA-based sequencing is by far the most widely used PCR methodology that is able to detect such uncultivable pathogens. However, this assay actually has some limitations linked to potential problems of contamination, which hampers diagnosis. To circumvent this, we have devised a simple P...

The thermosensitivity of the Escherichia coli dnaR130 mutant in initiation of DNA replication was suppressed by a spontaneous rifampin resistance mutation in rpoB, the gene for the beta subunit of RNA polymerase. Among the dnaR-suppressing rpoB alleles obtained was rpoB22, which was able to suppress the thermosensitivity of the dnaA46 or dnaA167 mutant, but not that of the dnaA5 mutant, in init...

UNLABELLED Fitness costs play a key role in the evolutionary dynamics of antibiotic resistance in bacteria by generating selection against resistance in the absence of antibiotics. Although the genetic basis of antibiotic resistance is well understood, the precise molecular mechanisms linking the genetic basis of resistance to its fitness cost remain poorly characterized. Here, we examine how t...

This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Multidrug-resistant (MDR) strains of Mycobacterium tuberculosis represent an obstacle in tuberculosis control and stress the need for rapid and reliable diagnostic tools for drug sus...

Background: Rifampicin resistant tuberculosis is a serious problem faced by control in China, and rapid detection of rifampicin resistance urgently needed. Objectives: This study aimed to describe the molecular characteristics frequency RNA polymerase β subunit (rpoB) gene mutations rifampicin-resistant (RR-TB) Anqing area. Methods: The rpoB fragment was amplified chain reaction (PCR), all isol...

the increasing incidence of multi drug resistance tuberculosis (mdr-tb) and extensively drug resistance tb (xdr-tb) worldwide highlight the urgent need to search for newer anti-tuberculosis compounds. it has been determined that pharmaceutical plant, hops ( humulus lupulus ), possesses some antibacterial effect. in this study, the antimycobacterial effect of this plant on rifampin sensitive and...