Abstract Roses ( Rosa hybrida ) are a highly merchandised flower but lack blue varieties. Overexpression of the flavonoid 3′,5′-hydroxylase F3′5′H gene can increase accumulation pigment (delphinidin anthocyanin). However, sometimes effect alone is inadequate for producing flowers. Furthermore, internal environment cell, such as an in pH, also help conversion anthocyanins to pigments. Nonetheles...