Abstract Kale ( Brassica oleracea convar. acephala var. sabellica ) is one of the oldest vegetable varieties with highest nutritional value and stress resistance. This work aimed to establish an efficient kale micropropagation protocol. Shoot tips were used as explants during axillary shoots multiplication. The most effective cytokinin (6 10 per explant after 1st 2nd passage, respectively) was ...

Cotyledonary leaf from 12 days old aseptically grown seedlings were used as explant. explants of two BINA varieties tomato cultured on MS medium containing different concentrations and combinations BAP, Kn IAA for multiple shoot formation via organogenesis. 100% induction was observed when the supplemented with IAA. Earliest within four weeks. The media 1.5 mg/l BAP + 0.5 0.2 found to be best r...

The influences of flax lignin and phenolic compounds obtained from spruce bark on the development of Lycopersicon esculentum plantlets were evaluated. Depending on the applied treatment and the concentrations used, the natural aromatic compounds had stimulatory effects on the germination capacity and the plantlets height and leaf area. The influence of lignin on Lycopersicon esculentum was lowe...

Tissue culture techniques can be beneficial for quick clonal propagation and production of disease-free plants of purple passion fruit as one of the most important medicinal and ornamental plants. These techniques are essential tools for the production of transgenic plants and high-value phytochemicals. The present study was planned to introduce an efficient in vitro propagation for pu...

A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot prim...

Genetically modified crops which had been commercial applied extensively majorly are the insect resistance and herbicide tolerance events. In this study, the Bt insecticidal gene Cry1Ab, the glyphosate-tolerant gene EPSPS, and the selection marker gene PMI were combined into a single transferred DNA fragment and introduced into sugarcane by Agrobacterium-mediated transformation. Thirty-three re...

Ranunculus wallichianus is a medicinally important plant and an endemic species to the Western Ghats of South India. An efficient reliable indirect regeneration protocol system for R. was developed from leaf explants in present investigation. Leaf were cultured on both full-strength half-strength MS (Murashige & Skoog) medium supplemented with different concentrations (0.5 mg L− 1 3.0 1) 2,4-D ...

In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26 degrees C) and low (4 degrees C) temper...

Grapevine (Vitis vinifera L.) is rich in phenylpropanoid compounds, namely flavonoids and stilbenes which, present in most tissues, are described as antioxidants and known to accumulate in response to biotic and abiotic stress. Grapevine is then a choice model for studying the interplay between the phenylpropanoid pathway and nutrient deficiency. Here we report the response to sulfur deficiency...

Rhododendron decorum is a woody species with high ornamental and medical value. Herein, we introduce novel in vitro regeneration method for R. decorum. We used flower buds to develop an efficient rapid plant protocol. Sterile of 2 cm size were as explants study the effects culture medium growth regulators on callus induction adventitious shoot differentiation, proliferation, rooting. According ...