نتایج جستجو برای: rna purification
تعداد نتایج: 304817 فیلتر نتایج به سال:
A procedure is presented for the purification of specific mRNAs, which exploits the ability of antibodies prepared against a native protein to bind to the nascent polypeptide on the polysome. Rather than precipitating these soluble antibody-polysome complexes with anti-antibody, which can lead to nonspecific trapping of polysomes, we have linked the anti-antibody to an insoluble matrix. Thus, t...
Replication of Viral Rna. Iv. Properties of Rna Synthetase and Enzymatic Synthesis of Ms2 Phage Rna.
Evidence has been presented' for the accumulation of a double-stranded replicative form of MS2 RNA in vivo, following infection of Escherichia coli with MS2 phage, and for the identity of this material with the double-stranded RNA which is labeled in vitro with radioactive carbon2 on incubation of CL4-labeled ribonucleoside triphosphates with RNA synthetase.3 The present paper is concerned with...
Abstract Background As wild poliovirus is eradicated, preventing circulation of vaccine-derived top priority. Our lab developed real-time multiplex PCR assays and deep sequencing methodology to detect characterize OPV strains from stool samples. The method requires gel purification product created viral RNA in However, the process filters out a significant portion Here, we compare gel- vs. DNA-...
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