The blast fungus Magnaporthe oryzae threatens global food security through the widespread destruction of cultivated rice. Foliar infection requires a specialized cell called an appressorium that generates turgor to force a thin penetration hypha through the rice cuticle and into the underlying epidermal cells, where the fungus grows for the first days of infection as a symptomless biotroph. Und...

Abstract Background Although panicle blast is more destructive to yield loss than leaf in rice, the cloned genes that function resistance are still very limited and molecular mechanisms underlying remain largely unknown. Results In present study, we have confirmed three Oxalate oxidase ( OXO ) genes, OsOXO2 , OsOXO3 OsOXO4 from a blast-resistant cultivar BC10 rice. The expression of were induce...

Rice blast disease is one of the most destructive rice diseases worldwide. The pi21 gene confers partial and durable resistance to Magnaporthe oryzae. However, little is known regarding the molecular mechanisms of resistance mediated by the loss-of-function of Pi21. In this study, comparative transcriptome profiling of the Pi21-RNAi transgenic rice line and Nipponbare with M. oryzae infection a...

Blast caused by Pyricularia oryzae is one of the major constraints limiting rice production globally. Study was undertaken at TNAU, India using a set of selected genotypes including cultivated varieties and (Near Isogenic Lines) NILs and they were screened for blast resistance both genotypically and phenotypically. Genetic diversity was studied among the genotypes and those which are diverse in...

Rice blast, caused by the fungus Pyricularia oryzae L., is considered one of main threats to world rice production. The development resistant cultivars best and sustainable control alternatives. Plant breeding efforts have been accelerated genetic mapping (linkage associative) marker assisted selection. On other hand, genomic editing techniques, such as meganucleases (MNs), Zinc-finger nuclease...

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent alleles of AVR-Pita. Using a map-based cloning strategy, we cloned Pi-ta, which is linked to the centromere of chromosome 12. Pi-ta encodes a predicted 928-amino acid cytoplasmic receptor with a centrally localized nucleotide binding site. A single-c...

Biotrophic invasive hyphae (IH) of the blast fungus Magnaporthe oryzae secrete effectors to alter host defenses and cellular processes as they successively invade living rice (Oryza sativa) cells. However, few blast effectors have been identified. Indeed, understanding fungal and rice genes contributing to biotrophic invasion has been difficult because so few plant cells have encountered IH at ...

Rice blast resistant gene, Pi54 cloned from rice line, Tetep, is effective against diverse isolates of Magnaporthe oryzae. In this study, we prospected the allelic variants of the dominant blast resistance gene from a set of 92 rice lines to determine the nucleotide diversity, pattern of its molecular evolution, phylogenetic relationships and evolutionary dynamics, and to develop allele specifi...

Molecular markers are useful for the identification of critical genes controlling agricultural traits of interest in crop germplasm and for the use of these genes in crop improvement using marker-assisted selection (MAS). The improvement of blast disease resistance of rice (Oryza sativa L.) varieties is one of the major goals for rice breeders. The blast resistance (R) gene Pi66(t) was previous...

in order to develop a high yielding rice cultivar with optimum cooking quality, a cross was conducted between two rice cultivars khazar, high yielding and resistant to blast disease and daylamani tarom, with good cooking quality in 1990. forty pure lines were selected and compared for high grain yield in 1996. ten selected lines together with two check cultivars dasht and onda were tested in ad...