Immunization with DNA can induce humoral and cell-mediated immune responses, both of which are important in conferring immunity to Toxoplasma gondii. The efficacy of genetic vaccination with a cDNA encoding the T. gondii SAG1 (P30) surface antigen was evaluated. Sera of immunized mice showed recognition of T. gondii tachyzoites by immunofluorescence and exhibited high titers of antibody to SAG1...

Tetranychus macfarlanei is a potential threat to various economically important crops. Chemical control widely used this spider mite, but the exclusive reliance on chemical acaricides now being questioned, and entomopathogens are emerging as promising alternative. The efficacy of largely depends abiotic factors like temperature, humidity, rainfall etc. experiments were conducted determine effec...

BACKGROUND Invasive aspergillosis (IA) and mucormycosis contribute to substantial mortality, especially among immunocompromised persons, including those with hematopoietic stem cell transplant (HSCT), hematologic malignancy (HM), and solid organ transplant (SOT). METHODS Using International Classification of Diseases, Ninth Revision codes available in the National Inpatient Sample, a hospital...

Mesenchymal stromal cells (MSCs) have recently been shown to play important roles in mammalian host defenses against intracellular pathogens, but the molecular mechanism still needs to be clarified. We confirmed that human MSCs (hMSCs) prestimulated with IFN-γ showed a significant and dose-dependent ability to inhibit the growth of two types of Toxoplasma gondii [type I RH strain with green flu...

Total Cerebral Blood Flow in the Monkey Measured by Hydrogen Clearance • Simple hydrogen-sensitive polarographical electrodes of thin platinum wire were inserted into the torcular Herophili of Rhesus monkeys. Hydrogen was administered by inhalation for ten minutes, after which the hydrogen clearance was recorded from torcular blood. At a Paco, of 32 mm Hg (SD ± 2.3), flow in the fast flow compa...

In cool conditions, physiologic markers accurately predict endurance performance, but it is unclear whether thermal strain and perceived thermal strain modify the strength of these relationships. This study examined the relationships between traditional determinants of endurance performance and time to complete a 5-km time trial in the heat. Seventeen club runners completed graded exercise test...

The aims of the present study were to diagnose toxoplasmosis in pregnant ewes and women serologically and molecularly, treat naturally infected ewes, and diagnose congenital toxoplasmosis. Blood samples were taken from 30 and 60 pregnant ewes and women, respectively, and used for diagnosis of toxoplasmosis through Latex agglutination test (LAT). Seropositive samples were confirmed by PCR for de...

Toxoplasmosis is one of the most prevalent parasitic infections common among humans and animals having two acute and chronic phases related to IgM and IgG, respectively. Immunoblotting is one of the serological methods with high sensitivity and specificity. Therefore, the present study aims to evaluate T. gondii RH strain antigen using the Western blot assay, their applications to distinguish b...

In the first report on the chemical structure of a nonmammalian LH-RH, chicken hypothalamic LH-RH was demonstrated to be [Gln8]LH-RH [2-4]. However, these studies and subsequent reports [7,8] did not totally exclude the possibility of a reverse sequence of the two amino acids Leu-Gln. In view of the recently described structure of salmon brain LH-RH as [Trp7,Leu8]LH-RH [9], we undertook to conf...

background: toxoplasma gondii is an obligatory intracellular protozoan parasite on which studies are pending regarding production of vaccine. to date, the production of human vaccine has not been successful where approximately one third of the world’s population is thought to be infected with t. gondii. objectives: the present study was designed to compare the electrophoretic patterns of t. gon...