The quality of any scientific data is directly proportional to that of the original starting samples, or simply ‘garbage in, garbage out’. In most circumstances it is logical to work with the highest quality material possible. However, for some experiments the highest quality possible is still a serious compromise from perfection. The degree to which the standard of input material influences fi...

The use of reverse transcription polymerase chain reaction (RT-PCR) with internal RNA competitive standards (competitors) provides a means for measuring absolute amounts of mRNA transcripts in small numbers of cells. Most quantitative competitive (QC)-RT-PCR methods require analysis of multiple reactions to determine the equimolar point of the products produced from mRNA vs. competitor RNA. Her...

Combining the patch-clamp method with single-cell reverse transcription polymerase chain reaction (scRT-PCR) a fusicoccin-induced current reflecting the activity of the plasma membrane H(+) ATPase of lily pollen protoplasts was measured and subsequently, the ATPase-encoding mRNAs were collected and amplified. Southern blot signals were observed in all 'patch-catch' experiments and could be dete...

BACKGROUND The insulin-like growth factor (IGF) system is important for pituitary development and control, with each member of this axis having a specific temporal and spatial expression. Because IGF-binding protein-5 (IGFBP-5) is one of the most highly expressed binding proteins in the anterior pituitary (AP), it might be of special importance in this gland. OBJECTIVE The purpose of this stu...

Real-time reverse transcription polymerase chain reaction (RT-PCR) has gained wide popularity as a sensitive and reliable technique for mRNA quantification. The development of new mathematical models for such quantifications has generally paid little attention to the aspect of error propagation. In this study we evaluate, both theoretically and experimentally, several recent models for relative...

BACKGROUND MUC1 is a membrane-bound mucin with an extensively O-glycosylated core protein and is developmentally regulated and aberrantly expressed by carcinomas. A high level of MUC1 mucin expression and secretion is associated with high metastatic potential and a poor prognosis. We studied the expression of MUC1 messenger ribonucleic acid (mRNA) in stage I lung adenocarcinoma by reverse trans...

Thousands of Caspian seals (Phoca caspica) died in the Caspian Sea from April to August 2000. Lesions characteristic of morbillivirus infection were found in tissue specimens from dead seals. Canine distemper virus infection was identified by serologic examination, reverse transcriptase- polymerase chain reaction, and sequencing of selected P gene fragments. These results implicate canine diste...

Using oral swab samples to detect West Nile virus in dead birds, we compared the Rapid Analyte Measurement Platform (RAMP) assay with VecTest and real-time reverse-transcriptase-polymerase chain reaction. The sensitivities of RAMP and VecTest for testing corvid species were 91.0% and 82.1%, respectively.

INTRODUCTION This study investigated influenza activity in Bangkok, Thailand between June 2009 and July 2012. METHODOLOGY Real-time reverse transcription polymerase chain reaction (RT-PCR) was performed to detect influenza viruses among patients with influenza-like illnesses. RESULTS Of the 6417 patients tested, influenza virus infection was detected in 42% (n = 2697) of the specimens. Infl...

Kumari S. (2009): Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RTPCR. Plant Protect. Sci., 45: 140–143. A one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The protocol was used to test infected screen house pl...