A variety of factors that influence real-time reverse transcriptase (RT)-PCR results were examined. Realtime reaction volumes can be decreased from the manufacturers suggested volumes (50μl) to as little as 5μl. Errors introduced through volume handlings can be significantly mitigated through the use of electronic pipettes. Random hexamers are compared against the use of a specific reverse tran...

Kawasaki disease (KD) is a self-limited, systemic vasculitis of children for which an infectious trigger is suspected. Recently, an association between KD and human coronavirus (HCoV)-New Haven (NH) was reported, on the basis of polymerase chain reaction (PCR) with primers that also amplified HCoV-NL63. We investigated the possible association between these HCoVs in the respiratory tract and KD...

Introduction: Accurate diagnosis of the COVID-19 disease is important. Currently, chest computed tomography (CT) and reverse polymerase chain reaction (RT-PCR) are being used for disease. This study was performed to evaluate Chest diagnostic value in comparison with RT - PCR method among patients. Methods: cross-sectional on suspected cases Imam Khomeini Hospital, Jiroft, Iran. Studied patients...

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...

abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction   (ic-rt-pcr) indicated that pepper isol...

Background: In the current COVID-19 pandemic, there is a rising need for a rapid and reliable diagnostic tool. We hypothesized that chest computed tomography (CT) can be a potential alternative for reverse transcription-polymerase chain reaction (RT-PCR). The aim of this study was to compare the diagnostic value of chest CT and RT-PCR in Iranian patients with suspected COVID-19. Methods: In a...

enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...

bovine viral diarrhoea virus (bvdv) is an important pathogen of dairy cattle. in this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of mashhad- iran, were tested for presence of bvdv by the use of a nested reverse transcription polymerase chain reaction (nested rt- pcr) assay. non of the cows in the herds had been vaccinated against...

Raspberry ringspot virus (RpRSV), which has not occurred in Japan, infects many plants such as raspberry, gooseberry, cherry, grapevine, and strawberry. Biological assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) have been introduced for the detection of RpRSV in postentry quarantine in Japan. We studied reverse transcription loop-me...

Abstract Rapid tests for active SARS-CoV-2 infections rely on reverse transcription polymerase chain reaction (RT-PCR). RT-PCR uses of RNA into complementary DNA (cDNA) and amplification specific (primer probe) targets using (PCR). The technology makes rapid identification the virus possible based sequence homology nucleic acid is much faster than tissue culture or animal cell models. However t...