Liquid chromatography-mass spectrometry (LC-MS, or alternatively HPLC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. LCMS is frequently used in drug development at many different stages including Peptide Mapping, Glycoprotein Mapping, Natural Products Derepli...

Recently, H. Kasai reported an automatic, precise method of 8-hydroxydeoxyguanosine (8-OH-dG) analysis in urine by high performance liquid chromatography coupled to an electrochemical detector (HPLC-ECD). It is based on a cleaning-up step by anion-exchange chromatography and a further purification step using reverse phase chromatography before detection, by the ECD. In this communication, we re...

Our objective was to establish a system for the investigation of neuropeptide biosynthesis in an insect. To achieve this we developed an in vitro organ culture system for the corpora cardiaca (CC) of the locust (Schistocerca gregaria). The CC are the neurosecretory structures containing the adipokinetic hormones AKH I and AKH II. Tritiated amino acids were added to the glands, and we studied th...

We describe a chemical assay involving "high-pressure" liquid chromatography for the quantitative determination of three aminoglycosides: netilmicin, tobramycin, and gentamicin. The drugs are separated from serum by means of precipitation of the serum proteins with acetonitrile after dilution with a buffer. The aminoglycosides are quantitatively extracted into the supernate, which is further pu...

We describe a reproducible protocol to explore for the first time the phosphoproteome of a seedless plant, the moss Physcomitrella patens. Following tryptic digestion of a total protein extract, phosphorylated peptides were isolated using the combination of C18 reverse-phase chromatography (RP-C18), immobilized Fe(3+) metal affinity chromatography (IMAC), capillary zone electrophoresis (CZE), l...

A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of Afloqualone in tablet and serum. Chromatography was performed by Shimadzu Model LC-20 ATVP with Kromasil C-18 column 250 x 4.6 mm ID with 5  particle sizes and the column maintained at ambient temperature. The injection volume was 20l and the total ...