Although evolutionary studies of gene function often rely on RNA interference, the ideal approach would use reverse genetics to create null mutations for cross-species comparisons and forward genetics to identify novel genes in each species. We have used transcription activator-like effector nucleases (TALENs) to facilitate both approaches in Caenorhabditis nematodes. First, by combining golden...

Reverse genetics systems have been established for all major groups of plant DNA and positive-strand RNA viruses, and our understanding of their infection cycles and pathogenesis has benefitted enormously from use of these approaches. However, technical difficulties have heretofore hampered applications of reverse genetics to plant negative-strand RNA (NSR) viruses. Here, we report recovery of ...

Handbook of Neurobehavioral Genetics and Phenotyping, First Edition. Edited by Valter Tucci. © 2017 John Wiley & Sons, Inc. Published 2017 by John Wiley & Sons, Inc. Cognitive neurogenetics is a genetics approach to the cellular and molecular neurobiology of cognitive mechanisms. It is predicated on the discovery (forward genetics)or production (reverse genetics) of mutant strains with a geneti...

Most of the genes of an organism are known from sequence, but most of the phenotypes are obscure. Thus, reverse genetics has become an important goal for many biologists. However, reverse-genetic methodologies are not similarly applicable to all organisms. In the general strategy for reverse genetics that we call TILLING (for Targeting Induced Local Lesions in Genomes), traditional chemical mut...

Most of the genes of an organism are known from sequence, but most of the phenotypes are obscure. Thus, reverse genetics has become an important goal for many biologists. However, reverse-genetic methodologies are not similarly applicable to all organisms. In the general strategy for reverse genetics that we call TILLING (for Targeting Induced Local Lesions in Genomes), traditional chemical mut...

The concept of reversed — or, as I prefer to call it, reverse — genetics was first formulated by Charles Weissmann in 1978 (Trends Biochem Sci 1978, 3:N109). In this form of genetics, a nucleic acid was modified at a predetermined position in vitro, and the phenotypic effects of this mutation were then assayed either in vitro or in vivo. In contrast, the classical form of genetics relies on fir...

Emerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic diarrhea virus (PEDV), deltacoronavirus (PDCoV), acute syndrome-CoV (SADS-CoV), cause severe in neonatal piglets, CoV infection is associated with significant economic losses for the industry worldwide. Reverse genetics systems realize manipulation of RNA genome facilitate development new vaccines. Thus far, five re...