Background: Secretory proteins of IgE receptor activated mast cells and basophils play a pivotal role in the generation of immediate and long term immune responses in allergy and type I hypersensitivity. Objective: The present study aims to generate a 2-D map and profile of proteins secreted from a high secretory variant of the rat basophilic leukemia cell line, RBL-2H3.1, which in view of the ...

Natural killer (NK) cells serve as one of the first lines of defense against viral infections and transformed cells. NK cell cytotoxicity is not dependent on antigen presentation by target cells, but is dependent on integration of activating and inhibitory signals triggered by receptor-ligand interactions formed at a tight intercellular contact between the NK and target cell, i.e., the immune s...

Few bacterial enzymes have been the subject of as much contention with regard to cofactor requirements as has aspartase. Since Gale's (1938) proposal of the two aspartases, it has become increasingly clear that much of the difficulty has arisen from the extrapolation of resting cell effects, based on ammonia data, to cell-free enzyme. The demonstration by Smith and Lichstein (1954) and by Trudi...

An in vitro preparation of the salamander olfactory epithelium has been developed for electrophysiological analysis. Intracellular measurements of membrane properties of the main epithelial cell types have been carried out, combined with Lucifer Yellow injections. The most prevalent type of cell had a high resting membrane potential and relatively low input resistance. This cell never discharge...

We investigated whether human resting T cells could be activated to proliferate and display effector function in the absence of T cell receptor occupancy. We report that combination of interleukin 2 (IL-2), tumor necrosis factor alpha, and IL-6 activated highly purified naive (CD45RA+) and memory (CD45RO+) resting CD4+ T cells to proliferate. Under this condition, memory resting T cells could a...

1. The pathogenesis of the mental retardation in phenylketonuria remains obscure. Leucocytes have proved of value in the study of other inborn errors of metabolism. The lymphocyte is a suitable model cell for the study of mammalian metabolism, because of its ability to divide in vitro in response to various stimuli. 2. We have examined the effects of phenylalanine, phenylpyruvate, phenyl-lactat...

Resting CD4 + T cells latently infected with HIV are a major barrier to a cure and therefore antiretroviral therapy is required life-long. There are two major potential pathways leading to the establishment of latency. One pathway, which we refer to as postactivation latency, occurs with the reversion of an activated infected CD4 + T cell to the resting memory state with an integrated silenced ...

In the absence of antigen, memory T helper (Th) cells are maintained in a resting state. Recently it has been shown that bone marrow (BM) is a major reservoir of resting memory Th cells. In a given immune response, less than 10% of the activated CD4 T cells are recruited to the pool of resting BM memory Th cells. Here we review recent evidence that CD69 and CD49b control homing of memory Th cel...

PURPOSE We have previously demonstrated that the AgNOR count in proliferating cells is a predictor of tumor recurrence in superficial bladder tumor (J. Urol. 162 (1999), 63-68). In the present study, we evaluate the type of AgNOR associated with cell cycles as a prognostic factor in invasive bladder tumor using a double staining technique employing both AgNOR and MIB-1 labelling. MATERIALS AN...