the present study was aimed to test the efficacy of adding vitamins c or e to tris-fructose-egg yolk diluent to increase awassi ram sperm storage period at 5 ˚c. semen samples from six mature awassi rams were used in this study. the semen samples were diluted by tris-glucose-egg yolk. diluted semen sample was divided into three parts. the first part was added with 0.9 mg ml-1 vitamin c, the sec...

to determine the effect of different levels of l-glutamine and glycerol on freezing coated sperm, semen were collected from 4 taleshi rams through artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. ejaculates were pooled, centrifuged and supernatant was removed, then cell fractions were used. samples were split into 9 equal parts and 3 (g3), 5 (g5) or 7% (g7) glycerol ...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...

The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL-1 vitamin...

background: the predominant phytoestrogen in soy and derived products is the isoflavone genistein. genistein has antioxidant properties. morphine is a main psychoactive chemical in opium that can increase the generation of free radicals and therefore it could adversely affects the spermatogenesis. objective: the main goal was to investigate whether the genistein could protect morphine adverse e...

experiment was conducted to study the effect of different antibiotics on sperm quality of ram by a completely randomized block design using 23 factorial arrangement with 2 block and 4 observations in each experimental units. factors include three antibiotics named gentamycin (g), lincomycin (l) and ampicillin (a) that were used at two different levels including zero and, respectively 5, 10 and ...