A gene-trapping vector carrying a GUS/Luciferase dual reporter gene was developed to establish an efficient and convenient screening system for T-DNA-based gene trapping in plants. A key feature of this gene trap scheme is to place two different types of reporters, luciferase (Luc) and beta-glucuronidase (GUS), as a fusion protein within a trapped gene to probe the activity of the gene. Luc is ...

Cas endonuclease-mediated genome editing provides a long-awaited molecular biological approach to the modification of predefined genomic target sequences in living organisms. Although cas9/guide (g)RNA constructs are straightforward assemble and can be customized virtually any site plant genome, implementation this technology cumbersome, especially species like triticale that difficult transfor...

Periodontitis is a frequent chronic inflammatory disorder destroying periodontium. Recent studies have revealed the role of bromodomain and extraterminal domain inhibitor (BETi) microRNA (miR)-130a in regulating macrophage polarization pro-inflammatory response. However, little known about whether apabetalone (a novel BETi) miR-130a are correlated with state periodontitis by polarization. Here ...

Conventional antimycobacterial screening involves CFU analysis, which poses a great challenge due to slow growth of mycobacteria. Recombinant strains carrying reporter genes under the influence of constitutive promoters allow rapid and wide screening of compounds but without revealing their modes of action. Reporter strains using pathway-specific promoters provide a better alternative but allow...

Silencing XB130 inhibits cell proliferation and epithelial‑mesenchymal transition in non‑small lung cancer (NSCLC), suggesting that downregulating expression may impede NSCLC progression. However, the molecular mechanism underlying regulation of remains unclear. In present study, role 3'‑untranslated region (3'‑UTR) was investigated. Recombinant psiCHECK‑2 vectors with wild‑type, truncated, or ...

Cuticular protein genes are good models to study the molecular mechanisms of signaling by ecdysteroids, which regulate molting and metamorphosis in insects. The present research demonstrates on hormonal regulation and analysis of the regulatory sequences and transcription factors important for Bombyx mori cuticular protein glycine-rich13 (CPG13) gene expression. Expression of CPG13 was strong a...

In our recent paper, we reported an aminoglycoside-sensing RNA in the leader RNA of the aminoglycoside acetyl transferase (AAC) and aminoglycoside adenyl transferase (AAD), enzymes that confer resistance to aminoglycoside antibiotics through modification of the drugs. Our study explains a well-known phenomenon: the induction of expression levels of the AAC/AAD proteins on addition of aminoglyco...

objective: in an attempt to develop safer and more effective gene therapy approaches as a realistic treatment for various forms of cancer, researchers are increasingly using tumor-specific promoters (tsp) to drive the expression of the gene of interest and eradicate cancer cells. in this study, for the first time we introduce the oct-4 promoter as a cancer-specific promoter with a high efficacy...