The Escherichia coli Rep protein is a 3' to 5' SF1 DNA helicase required for replication of bacteriophage phiX174 in E. coli, and is structurally homologous to the E. coli UvrD helicase and the Bacillus stearothermophilus PcrA helicase. Previous crystallographic studies of Rep protein bound to single-stranded DNA revealed that it can undergo a large conformational change consisting of an approx...

Cotton leaf curl Burewala virus (CLCuBuV), belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP) genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV) were fused with β-glucuronidase (GUS) and green f...

Adeno-associated virus type 2 (AAV-2), a human parvovirus which is apathogenic in adults, inhibits replication and gene expression of human immunodeficiency virus type 1 (HIV-1) in human cells. The rep gene of AAV-2, which was shown earlier to be sufficient for this negative interference, also down-regulated the expression of heterologous sequences driven by the long terminal repeat (LTR) of HI...

PURPOSE Choroideremia (CHM) is an X-linked ophthalmic disease. The gene associated with CHM (REP-1) encodes a ubiquitously expressed protein that is indispensable for the posttranslational activation of retina-specific Rab protein. Different mutations, including large genomic rearrangements involving the REP-1 gene, are responsible for CHM, but they all cause the protein to be truncated or abse...

The red edge position (REP) has been widely used to estimate vegetation parameters and also as a sensitive indicator of vegetation stress. Detailed characterizations of REP can be easily achieved from hyperspectral data obtained from imaging spectrometers. After launch of imaging spectrometers, shift in the center wavelength of the spectral channel may occur due to the vibrations and aging of c...

Associative learning produces conditioned stimulus (CS)-specific plasticity of frequency receptive fields (RFs) in the auditory cortex; responses to the CS frequency are increased, whereas responses to other frequencies are decreased. This study determined the effects of habituation on the RF of neurons in the auditory cortex of the guinea pig (Cavia porcellus). One frequency was presented repe...

We define and study the category Rep(Q, F1) of representations of a quiver in Vect(F1) the category of vector spaces ”over F1”. Rep(Q, F1) is an F1–linear category possessing kernels, co-kernels, and direct sums. Moreover, Rep(Q, F1) satisfies analogues of the Jordan-Hölder and Krull-Schmidt theorems. We are thus able to define the Hall algebra HQ of Rep(Q, F1), which behaves in some ways like ...

tarkhineh is one of the traditional foods in iran and a rich source of probiotic bacteria. the objective of this study was to identify the probiotic bacteria isolated from tarkhineh using 16s rrna gene sequencing and molecular typing with repetitive extragenic palindromic–pcr (rep-pcr). in total, 20 different bacteria were isolated from traditional dairy products and tarkhineh. molecular identi...