Cytokinesis is a dramatic event in the life of any cell during which numerous mechanisms must coordinate the legitimate and complete mechanical separation into two daughter cells. We have used Dictyostelium discoideum as a model system to study this highly orchestrated event through genetic analysis. Transformants were generated using a method of insertional mutagenesis known as restriction enz...

BACKGROUND Despite efforts to establish uniform protocols for the management of severe sepsis, this condition continues to have high morbidity and mortality. This is due, among other factors, to the many barriers for the development of the protocols and the application time. That is why new therapeutic measures are continuing to be investigated and developed. OBJECTIVE To review the literatur...

Submitted for the MAR08 Meeting of The American Physical Society Unusual aggregation behavior of colloids coated with palindromic DNA MIRJAM LEUNISSEN, REMI DREYFUS, DAVID PINE, PAUL CHAIKIN, New York University — Coating particles with single-stranded DNA ‘sticky ends’ gives excellent control over the specificity, strength and range of their interactions. Usually, a pair of complementary ‘Wats...

The social amoeba Dictyostelium discoideum, a powerful paradigm provides clear insights into the regulation of growth and development. In addition to possessing complex individual cellular functions like a unicellular eukaryote, D. discoideum cells face the challenge of multicellular development. D. discoideum undergoes a relatively simple differentiation process mainly by cAMP mediated pathway...

High mobility group box 1 (HMGB1) is a pivotal mediator of sepsis progression. Remifentanil, an opioid agonist, has demonstrated anti-inflammatory effects in septic mice. However, it is not yet known whether remifentanil affects the expression of HMGB1. We investigated the effects of remifentanil on HMGB1 expression and the underlying mechanism in septic rats. Forty-eight male Sprague-Dawley ra...

In their comment, Haanes et al., suggest that CGRP could act through two distinct receptors in human coronary arteries; namely the CGRP receptor (CLR/RAMP1) and the AMY1 receptor (CTR/RAMP1). 1 Although there are other possible explanations, the presence of multiple CGRP receptor subtypes could explain their elegant data. In light of the active clinical development of blocking antibodies and sm...

Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disrupted gene was denominated gdt1. The mutant phenotype was reconstructed by dis...

Ability of biocontrol agent Streptomyces griseus to control the F. oxysporum f. sp. lycopersici. (FOL) induced fusarium wilt disease in tomato was studied. Talc-based formulation of Streptomyces griseus either single or with or without chitin were developed and tested under greenhouse conditions. Self life of S. griseus in talk based formulation showed stable up to 105 day with 122 x 10 , 118 x...