Although much is known about the mechanisms of signal-mediated protein and RNA nuclear import and export, little is understood concerning the nuclear import of plasmid DNA. Plasmids between 4.2 and 14.4 kilobases were specifically labeled using a fluorescein-conjugated peptide nucleic acid clamp. The resulting substrates were capable of gene expression and nuclear localization in microinjected ...

Infection of non-dividing cells is a biological property of HIV-1 crucial for virus transmission and AIDS pathogenesis. This property depends on nuclear import of the intracellular reverse transcription and pre-integration complexes (RTCs/PICs). To identify cellular factors involved in nuclear import of HIV-1 RTCs, cytosolic extracts were fractionated by chromatography and import activity exami...

in recent decades, theorists proposed the role of domestic components such as interior active groups, policies and macroeconomic indicators on determination of protection policies. in the context of recent studies, this study has investigated the effect of business cycle fluctuations on import protection for selected developing countries in 1995-2011 by using dynamic panel data method. furtherm...

Nuclear import of some viral proteins depends on importin a proteins. However, no preferences of distinct a-importins for any viral protein import have been demonstrated. We used in vitro import assays and observed that all ubiquitously expressed human importin a isoforms mediate nuclear translocation of adenoviral E1A. Competition with nucleoplasmin suggests that importin a3 is the most effici...

The nuclear import of proteins typically requires the presence of a nuclear localization sequence (NLS). Some proteins have more than one NLS, but the significance of having multiple NLSs is unclear. The enzyme 5-lipoxygenase (5-LO) has three NLSs that, unlike the tight cluster of basic residues of the classical SV40 large T antigen NLS, contain dispersed basic residues. When attached to green ...

the analyses by the first ranked economists show that the economical growth of the countries that import oil or non-oil developing countries (nodcs) depends on the economical growth of the industrial countries. “goldstein” & “khan” by means of a long & complete verification show the dependence of the economical growth and “callier” also claims this fact that the economical growth of the countri...