To realize efficient homogeneous electrophoretic immunoassays, we introduce discontinuous polyacrylamide gels that enable quantitative assay completion in separation lengths as short as 350 mum in <10 s. The discontinuous cross-linked gels reduce the required electrophoretic separation lengths and thereby significantly reduce the required applied electrical potentials needed to achieve 100's V/...

sepsis is a significant health problem with an estimated 750,000 new cases in the usa annually. it is also the third leading cause of death in developed countries, equaling the number of fatalities from acute myocardial infarction. the high sepsis-related mortalities mean there is an urgent need to improve the diagnosis and management of sepsis patients. the aim of this study was the evaluation...

A unified modeling approach that combines rigorous rate-based balance equations with the model-order reduction properties of orthogonal collocation on finite elements (OCFE) approximation techniques is employed for the design and optimization of complex staged separation processes. The process model involves the rigorous description of mass and heat transfer phenomena, phase equilibrium, and ch...

Juvenile chronic myelogenous leukemia (JCML) is a myeloproliferative disease in which morbidity and mortality are primarily caused by nonhematopoietic organ failure from myelomonocytic infiltration or by failure of the normal bone marrow. Morphologic evidence of maturation arrest, karyotypic abnormalities, and progression to blast crisis are infrequent events. Viral infections and other reactiv...

The secure base and safe haven effects of the attachment figure are central features of the human attachment theory. Recently, conclusive evidence for human analogue attachment behaviours in dogs has been provided, however, the owner's security-providing role in danger has not been directly supported. We investigated the relationship between the behavioural and cardiac response in dogs (N= 30) ...

Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeli...

The industrial state of the art prescribes a physical separation of robotic manipulators and human workers by means of safety barriers, in order to prevent risks of collisions. This separation has a clear negative influence on the widespread use of robotic technology in shopfloors. On the other hand, the removal of protective barriers gives rise to safety issues, that can be addressed with a co...

Peroxynitrite (ONOO(-)) is a highly reactive species implicated in the pathology of several cardiovascular and neurodegenerative diseases. It is generated in vivo by the diffusion-limited reaction of nitric oxide (NO(*)) and superoxide anion ((*)O(2)(-)) and is known to be produced during periods of inflammation. Detection of ONOO(-) is made difficult by its short half-life under physiological ...

Optimization of performance of holographic gratings was investigated by changing the chemical structures of photopolymerizable monomers, the LC content, and recording beam intensity, which strongly related to the control of kinetics of polymerization and phase separation of LCs. High diffraction efficiency was obtained using 3-acryloxypropyltrimethoxysilane (APTMS) and 2-[(3,4-epoxycyclohexyl)e...