The DynaFit software package (http://www.biokin.com/dynafit/) contains a builtin (“hard-coded”) implementation of the integrated Michaelis-Menten equation formulated in terms of the Lambert omega function. This built-in regression model can be used conveniently to determine the substrate kinetic parameters either from a single progress curve or from a collection of enzymatic progress curves ana...

A difficulty associated with high throughput screening for enzyme inhibitors is to establish reaction conditions that maximize the sensitivity and resolution of the assay. Deduction of information from end-point assays at single concentrations requires a detailed understanding of the time progress of the enzymatic reaction, an essential but often difficult process to model. A tool to simulate t...

In the present investigation efforts have been made to synthesis polyvinyl pyridine (PvPy) /C60 conjugates through 2, 2-azobisisobutyronitrile (AIBN) initiated insitu polymerization of PvPy in scCO2.The process of synthesis was monitored under different reaction conditions ranging 1200-1800psi, 60-90 oC over 6 hours. The progress of polymerization was monitored rheoviscometrically. The concentr...

Although the advent of nonionic low-osmolar contrast agents has reduced the probability of a reaction to radiopaque contrast media derived from tri-iodinated benzoic acid, reactions still occur. Radiologic technologists must understand and know how to manage adverse effects of contrast media. Prompt attention to patients who exhibit the early signs of an adverse reaction can help to ensure the ...

An electrochemical cell coupled with a recycle loop through a transmission FTIR cell is employed in studies of two free radical organic reactions, the oxidation of allylic alcohols and the trifluoromethylation of heteroarenes. Rapid mixing through the recycle loop allows continuous monitoring of reaction progress. Electrochemical generation of free radicals allows their controlled mediation int...