نتایج جستجو برای: rdna 16s

تعداد نتایج: 31200  

Journal: :International journal of systematic and evolutionary microbiology 2001
H G Montano R E Davis E L Dally S Hogenhout J P Pimentel P S Brioso

Hibiscus rosa-sinensis is a valuable ornamental species widely planted in Brazil. Many plants are affected by witches' broom disease, which is characterized by excessive axillary branching, abnormally small leaves, and deformed flowers, symptoms that are characteristic of diseases attributed to phytoplasmas. A phytoplasma was detected in diseased Hibiscus by amplification of rRNA operon sequenc...

Journal: :The British journal of ophthalmology 2011
Sunao Sugita Norio Shimizu Ken Watanabe Miki Katayama Shintaro Horie Manabu Ogawa Hiroshi Takase Yoshiharu Sugamoto Manabu Mochizuki

AIM To measure the bacterial genome in ocular fluids and to analyse the clinical relevance of infectious endophthalmitis. METHODS Nineteen ocular fluid samples (eight aqueous humour and 11 vitreous fluid samples) were collected from 19 patients with suspected bacterial endophthalmitis. Fifty ocular samples from uveitis patients were also collected along with 40 samples from patients without o...

2013
Anna Klindworth Elmar Pruesse Timmy Schweer Jörg Peplies Christian Quast Matthias Horn Frank Oliver Glöckner

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs were evaluated in silico with respect to the SILVA 16S/18S rDNA non-redundant reference dataset (S...

2016
Wenping Wang Kun Zhang Daogui Deng Ya-Nan Zhang Shuixiu Peng Xiaoxue Xu

Increased human activities and environmental changes may lead to genetic diversity variations of Cladocerans in water. Daphnia pulex are distributed throughout the world and often regarded as a model organism. The 16S rDNA, cytochrome c oxidase subunit I (COI), and 18S genes were used as molecular marks. The genetic diversity and phylogeny of D. pulex obtained from 10 water bodies in the middle...

1997
NAOMI L. WARD-RAINEY

A new hierarchic classification structure for the taxa between the taxonomic levels of genus and class is proposed for the actinomycete line of descent as defined by analysis of small subunit (16s) rRNA and genes coding for this molecule (rDNA). While the traditional circumscription of a genus of the actinomycete subphylum is by and large in accord with the 16s rRNA/rDNA-based phylogenetic clus...

2012
Priit Kasenõmm Jelena Štšepetova

Background: The aim of the study was to analyze the performance of PCR-DGGE based assay and its applicability as a tool for the identification of bacteria in the middle ear of children with otitis media with effusion (OME). Methods: The middle ear effusions from 20 children with OME were analyzed both by bacterial culture and by 16S rDNA-gene-targeted PCR assay, DGGE fingerprinting and sequenci...

2011
Helena Sanches Marcon Virgínia Elias Coscrato Denise Selivon André Luiz Paranhos Perondini Celso Luis Marino

Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S ...

2016
Shutu Xu Qianqian Sun Xiaohua Zhou Xiao Tan Man Xiao Wei Zhu Ming Li

The 16S rDNA, 16S-23S rDNA-ITS, cpcBA-IGS, mcy gene and several polysaccharide biosynthesis-related genes (epsL and TagH) were analyzed along with the identification of the morphology of Microcystis colonies collected in Lake Taihu in 2014. M. wesenbergii colonies could be distinguished directly from other colonies using espL. TagH divided all of the samples into two clusters but failed to dist...

Journal: :Applied and environmental microbiology 1998
P Vinuesa J L Rademaker F J de Bruijn D Werner

We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic resolution by computer-assisted analysis of 16S ribosomal DNA (rDNA) PCR-restricti...

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 1997
J Hitti D E Riley M A Krohn S L Hillier K J Agnew J N Krieger D A Eschenbach

We amplified bacterial 16S rRNA encoding DNA (rDNA) with the polymerase chain reaction (PCR) to detect amniotic fluid infection in 69 women in premature labor whose membranes were intact. Bacterial rDNA was detected by PCR in samples from 15 (94%) of 16 patients with positive amniotic fluid cultures. Bacteria were detected by PCR in samples from 5 (36%) of 14 patients with negative cultures and...

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