To further understand the relationships between DNA damage, DNA repair, and cellular end points such as survival and mutation, the repair capacity of a DNA repair-deficient mutant (strain UV-20) of Chinese hamster ovary cells was characterized in response to DNA cross-linking agents. This mutant, previously shown to be hypersensitive to killing by both ultraviolet light and the cross-linking ag...

The clinical drug psoralen, in the presence of 360-nm light, binds to cellular DNA, forming monofunctional adducts with pyrimidine bases and interstrand cross-links. Cross-links, however, rather than monoadducts, appear to be the DNA lesion primarily responsible for the sensitizing action of this drug. Formation of an interstrand cross-link necessarily damages both DNA strands at nearly the sam...

To further understand the relationships between DNA dam age, DNA repair, and cellular end points such as survival and mutation, the repair capacity of a DNA repair-deficient mutant (strain UV-20) of Chinese hamster ovary cells was character ized in response to DNA cross-linking agents. This mutant, previously shown to be hypersensitive to killing by both ultra violet light and the cross-linking...

Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet. Structural basis for transcription coupled repair: the N-terminus of Mfd resembles UvrB with degenerate ATPase motifs. Journal of Molecular Biology 355(4): 675-683. "[One] topic we touched on was mutation ... We totally missed the possible role of … [DNA] repair although … I later came to realise that DNA is so precious tha...

Several important anti-tumor agents form DNA interstrand crosslinks (ICLs), but their clinical efficiency is counteracted by multiple complex DNA repair pathways. All of these pathways require unhooking of the ICL from one strand of a DNA duplex by nucleases, followed by bypass of the unhooked ICL by translesion synthesis (TLS) polymerases. The structures of the unhooked ICLs remain unknown, ye...