A-kinase anchoring proteins (AKAPs) function to target protein kinase A (PKA) to specific locations within the cell. AKAPs are functionally identified by their ability to bind the type II regulatory subunits (RII) of PKA in an in vitro overlay assay. We previously showed that follicle-stimulating hormone (FSH) induces the expression of an 80-kDa AKAP (AKAP 80) in ovarian granulosa cells as they...

Developing rat ova have been studied with the electron microscope. Special attention was paid to relations of ova to the granulosa cells, the developmental stages of ovarian follicles, and the cytology of the unfertilized tubal ova. The relationship of the oocyte to the surrounding granulosa cells was found to change from one of a simple apposition of the plasma membranes to a complex interdigi...

Background: Ovarian cancer is common amongst women worldwide. In Indonesia, it the fourth most female with a 5-year survival rate of 45%, but can reach up to 92% if treated early. Granulosa cell tumors (GCT) arise from sex-cord and are considered malignant. This study presents rare case asymptomatic adult-type granulosa tumor in an elderly lady diagnosed during routine pelvic ultrasound scannin...

Developing rat ova have been studied with the electron microscope. Special attention was paid to relations of ova to the granulosa cells, the developmental stages of ovarian follicles, and the cytology of the unfertilized tubal ova. The relationship of the oocyte to the surrounding granulosa cells was found to change from one of a simple apposition of the plasma membranes to a complex interdigi...

Ovarian follicular fluid peptide (OFFP) purified from sheep ovaries enhances apoptotic changes in ovarian granulosa cells of mice. To get an insight into the cell subpopulations responding to OFFP, the heterogeneity of granulosa cells was resolved. Subpopulations of granulosa cells were obtained from ovaries of immature mice treated with PMSG alone and autopsied 48 hr (control) and 72 hr after ...

Orphan nuclear receptors such as germ cell nuclear factor (GCNF), steroidogenic factor 1 (SF-1) and liver receptor homolog-1 (LRH-1), are emerging as important ovarian factors in regulating female reproduction. Within the ovary, GCNF (NR6A1) expression is restricted to the oocyte, while SF-1 (NR5A1) is expressed only in the somatic cells, such as granulosa, thecal and luteal cells, and intersti...

The production of ovarian neoplaslns in female mice by x-rays was described in 1936 (7, 10). q]ae tmnors arose about 7 to 10 months following irradiation with single or multiple doses of 200 r to 400 r. These tumors were of 3 histological types: (a) granulosa cell tumors, (b) luteomas, and (c) tubular adenomas. Whether the gland or duct-like structures of some of these adenomas are related to a...

The direct effect of insulin and insulin-like growth factor-Ion ovarian granulosa cell function in cattle was evaluated by using a serum-free culture system. Granulosa cells were obtained from small (1 to 5 mm) follicles collected from cattle and cultured for 4 days. Culture medium was collected for quantification of progesterone, and cell numbers were determined. Both insulin and IGF-I signifi...

We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other gra...