The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served...

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes w...

We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each...

Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK ...

هديكچ ط ي لاس اه ي خا ي ر پ ي تفرش لباق هظحلام ا ي رد طابترا اب لولس ه ا ي نب ي دا ي لصاح هدش تسا هک ون ي د شخب هار اهراک ي ون ي ن نامرد ي رد ب ي رام ي اه ي بعص جلاعلا م ي .دشاب ا ي ن لولس اه هک رد مامت ناگرا ي مس اه ي دنچ لولس ي روضح ،دنراد اناوت يي سقت ي م و دبت ي ل هب لولس اه يي سب ي را صاصتخا ي ار هتشاد و نچمه ي ن رداق هب اج ي زگ ي ن ...

The primary goal of this research was to reveal de novo mutations in the liquidators (cleanup personnel) who emigrated to Israel from the Chernobyl disaster area. We used genome fingerprinting simple sequence repeat-anchored polymerase chain reaction (PCR) amplification and random amplified polymorphic DNA PCR (RAPD PCR). The methodology involved a combination of RAPD PCR, polyacrylamide gel el...

The random-amplified polymorphic DNA (RAPD) technique (5,7) is one of the most useful methods for species identification and studies on the genetic structure of populations of microand macro-parasites. This method generally provides numerous markers that must be cloned and labeled to be used as probes (1,4). These probes can be used to test the specificity and the polymorphism of the RAPD marke...

The infection dynamics of metacercariae were assessed in cyprinoid fish (cyprinoids) from Chiang Mai and Sakon Nakhon Provinces, Thailand, during October 2008 to September 2009. The samples were collected during 3 seasons from rivers and local markets. Metacercarial infection was determined by acidpepsin digestion and confirmed using HAT-RAPD PCR method. Thirteen and 16 species of cyprinoids we...

صخلملا A total of 48 fresh water fishes, belonging to four species of the family Cyprinidae (Capoeta trutta, Chalcalburnus mossulensis, Cyprinion macrostomum and Leuciscus lepidus) were collected from Darbandikhan lake, southwest Sulaimaniya city, Kurdistan region, in the north of Iraq, from March to the end of July 2008. The inspection of gills revealed the infection of these fishes with four ...