Raman microspectroscopy is a label-free and nondestructive technique to measure the intrinsic chemical profile of single cells. The naturally weak Raman signals hampered the application of Raman spectroscopy for high-throughput measurements. Nearly all photosynthetic microorganisms contain carotenoids that are active molecules for resonance Raman at a 532 nm excitation wavelength. Hence, the ac...

Altangerel et al. (1) suggest that Raman spectroscopy can be used to detect the early abiotic stress response in plants through the measurement of anthocyanins and carotenoids in plant tissues. However, we believe there are some problems that need to be considered. First, there is insufficient evidence that Raman spectroscopy can be used to measure changes in the composition of plants during th...

In this study, we present a BCC detector based on confocal Raman spectroscopy using maximum a posteriori probability (MAP) and fuzzy algorithm. The preprocessing step consists of data normalization with minmax method and dimension reduction with principal components analysis (PCA). To enhance the classification performance, we divide Raman spectra into two significant protein bands, i.e., Amid ...

Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated b...

A novel strategy for the rapid detection and identification of traditional and emerging Campylobacter strains based upon Raman spectroscopy (532 nm) is presented here. A total of 200 reference strains and clinical isolates of 11 different Campylobacter species recovered from infected animals and humans from China and North America were used to establish a global Raman spectroscopy-based dendrog...

This work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800-1800 cm(-1)) and high-wavenumber (HW)(2800-3600 cm(-1)) Raman spectra from esopha...

The current study assessed the feasibility of the application of Raman spectroscopy toward the diagnosis of gout and pseudogout. First, the lowest concentrations of monosodium urate monohydrate (MSUM) and calcium pyrophosphate dihydrate (CPPD) crystals detectable by Raman spectroscopy were investigated by mixing known amounts of synthetic crystals with synovial fluid in the concentration range ...

Raman spectroscopy is the use of inelastic scattering of light to probe various properties of molecules and materials. Incoming photons interact with the electron cloud of the molecular bonds and inelastically scatter. During this scattering the energy of the incoming photon is shifted to red or blue depending on the particular process. This phenomenon is called Raman scattering and was discove...

Received June 11, 1984; accepted September 20, 1984 A time-resolved coherent anti-Stokes Raman technique is demonstrated that yields a spectral resolution beyond the linewidth obtained in spontaneous Raman spectroscopy. Two picosecond dye lasers, independently tunable with low timing jitter, are used. The coherent material excitation is generated by long pump pulses and monitored by short delay...

A major challenge in quantitative biological Raman spectroscopy, particularly as applied to transcutaneous Raman spectroscopy measurements, is overcoming the deleterious effects of scattering and absorption (turbidity). The Raman spectral information is distorted by multiple scattering and absorption events in the surrounding medium, thereby diminishing the prediction capability of the calibrat...