Resonance Raman spectroscopy (RRS) possesses many characteristics that are important for detecting, identifying and monitoring chemical effluents. Raman scattering is a coherent, inelastic, two-photon scattering process where an exciting photon of energy hv promotes a molecule to a virtual level and the subsequently emitted photon is shifted in frequency in accordance with the rotational-vibrat...

The goal of this study was to develop a compact fiber optic probe to measure near infrared Raman spectra of human cervical tissue in vivo for the clinical diagnosis of cervical precancers. A Raman spectrometer and fiber optic probe were designed, constructed and tested. The probe was first tested using standards with known Raman spectra, and then the probe was used to acquire Raman spectra from...

Graphene is a monolayer of carbon atoms packed into a two-dimensional (2D) honeycomb crystal structure, which is a special material with many excellent properties. In the present study, we will discuss the possibility that graphene can be used as a substrate for enhancing Raman signals of adsorbed molecules. Here, phthalocyanine (Pc), rhodamine 6G (R6G), protoporphyin IX (PPP), and crystal viol...

The resonance Raman effect has been known for many years’. Its potential for trace analysis was pointed out by Brandmiiller2 in 1959. However, full exploitation of resonance Raman spectrometry did not begin until the development of high power, multiline gas lasers as Raman excitation sources. The recent application of tunable dye lasers as excitation sources3 promises even broader applications ...

We present the method of theoretical calculations of the Raman intensities and the simulated Raman spectra of platinum(II) complexes. Theoretical Raman spectra of the anticancer agents: cisplatin (1), carboplatin (2), cis-[Pt(orotato)(NH3)2] (3), cis[PtCl2(NH3)(2-picoline)], ZD0473 (4), and the two transient species of 4 (the hydrolysis products) were calculated by density functional mPW1PW met...

Broadband coherent Raman generation emerges as a successful method to produce multicolored femtosecond pulses and time-shaped laser fields. In our study, coherent Raman sidebands are generated in a Raman-active crystal, driven by two-color femtosecond laser pulses. An interferogram of the sidebands based on coherent Raman scattering is produced in a novel reflection scheme. The relative spectra...

The application of Raman spectroscopy to characterize natively unfolded proteins has been underdeveloped, even though it has significant technical advantages. We propose that a simple three-component band fitting of the amide I region can assist in the conformational characterization of the ensemble of structures present in natively unfolded proteins. The Raman spectra of alpha-synuclein, a pro...

Raman spectroscopy is based on analysis of inelastically scattered light. This technique has a wide range of uses in chemistry, solid-state physics and other fields. Raman spectroscopy can distinguish between molecules and can identify them by their characteristic spectra. In this seminar I will discuss instrumentation required for Raman spectroscopy in medical applications, followed by specifi...

An optimized noninvasive Raman microscope was used to evaluate tumor targeting and localization of single walled carbon nanotubes (SWNTs) in mice. Raman images were acquired in two groups of tumor-bearing mice. The control group received plain-SWNTs, whereas the experimental group received tumor targeting RGD-SWNTs intravenously. Raman imaging commenced over the next 72 h and revealed increased...

The IlluminatIR™ is commonly used with either a diamond contact ATR (attenuated total reflection) or a diffuse reflectance objective. The sample can be viewed, and Raman spectra collected, either through these objectives or through the high quality microscope objectives supplied with the inVia Raman microscope. Simple switching between video view, IR acquisition, and Raman acquisition allows IR...