In a previous paper (l), the conversion of labeled pyruvate and of labeled glucose to glycogen, glucose, and COZ by rat liver slices, in vitro, was reported. It is the purpose of the present communication to present experiments on the metabolism of labeled glycerol and of labeled pyruvate by the same experimental system. The results provide quantitative information on the metabolic pathways fol...

The kinetics of the two purified forms of pyruvate kinase from Salmonella typhimurium LT-2 were studied in assays at pH 6.8 where the relationships between the initial velocities of the catalysed reactions and Mg2+ are non-hyperbolic. The analysis show that Mg2+ display positive homotropic interactions in their binding behaviour with Hill coefficient values of 2.5 and 1.2 for the form I and II,...

Buller, C. S. (University of Kansas, Lawrence), and J. M. Akagi. Hydrogenase of Coleman's sulfate-reducing bacterium. J. Bacteriol. 88:440-443. 1964.-The hydrogenase of the Coleman organism was found to be associated with the particulate fraction of cell-free extracts. This enzyme catalyzed the oxidation of molecular hydrogen in the presence of various electron acceptors. It was also able to ev...

HENDERSON, ANDREW H., ROBERT J. CRAIG, RICHARD GORLIN, AND EDMUND Iif. SONNENBLKK. Lactate and fyruvate kinetics in Z’SOla&perfused rat hearts. Am. J, Physiol. 2 17 (6) : 1752-I 756. 1969.Myocardial lactate and pyruvate kinetics have been studied by measuring their concentrations in arterial, venous, and tissue samples from isolated rat hearts perfused with different concentrations of single su...

Human malaria is attributed to five species of the Plasmodium parasite (this includes the discovery that P. knowlesi infects humans). The most virulent form of the disease is caused by P. falciparum, which continues to have a major impact on human populations: a third of the world’s population live in malaria endemic areas and upwards of a million individuals succumb to the disease each year, p...

Phosphoenolpyruvate carboxykinase (adenosine 5'-triphosphate) was the only enzyme capable of carboxylating pyruvate or phosphoenolpyruvate that could be demonstrated in sonicated cells or cell-free extracts of a group 1 butyrivibrio.

Octopine dehydrogenase (OcDH) from the adductor muscle of the great scallop, Pecten maximus, catalyzes the NADH dependent, reductive condensation of L-arginine and pyruvate to octopine, NAD(+), and water during escape swimming and/or subsequent recovery. The structure of OcDH was recently solved and a reaction mechanism was proposed which implied an ordered binding of NADH, L-arginine and final...

The pyruvate metabolism of LactobaciUus plantarum and Latobacillus arabino8su, which according to Bergey (1948) are identical organisms, has been studied by Rowatt (1951) and Nossal (1952). Both these homofermentative lactobacilli were shown to form 3-hydroxybutan-2-one (acetoin) as the main product of their pyruvate metabolism. No one appears to have studied the metabolism of pyruvate by any o...

Imaging of the metabolism of hyperpolarized [1-(13) C]pyruvate has shown considerable promise in preclinical studies in oncology, particularly for the assessment of early treatment response. The repeatability of measurements of (13) C label exchange between pyruvate and lactate was determined in a murine lymphoma model in fasted and non-fasted animals. The fasted state showed lower intra-indivi...