A fragment from the coding sequence of a human parathyroid hormone-related peptide (PTH-RP) cDNA (1-3) was used to isolate from a lambda gt-11 cDNA library prepared from 10 day old chicken embryo poly A + RNA (Clontech Laboratories Inc.) a 1411 base pair cDNA pcPLPl (bases 16-1436) which encodes the chicken homolog. In the sequence below, the underlined nucleotides (bases 1 —25) were determined...

A radioimmunoassay specific for the amino (N) terminal portion of the parathyroid hormone (PTH) molecule (N-PTH radioimmunoassay) has been developed by iodinating synthetic 1-34bovine PTH (1-34bPTH) and using commercially available bPTH antiserum. A radioimmunoassay specific for the carboxyl (C) terminal (C-PTH radioimmunoassay) has been carried out by adding enough amount of 1-34bPTH to the PT...

Parathyroid hormone (PTH) is involved in regulation of the calcium level in blood and has an influence on bone metabolism, thus playing a role in osteoporosis therapy. In this study, the structures of the human PTH fragments (1-34) and (1-39) as well as bovine PTH(1-37) in aqueous buffer solution under near physiological conditions were determined using two-dimensional nuclear magnetic resonanc...

The immunochemiluminometric assay described uses immobilized anti-human parathyrin (parathyroid hormone, hPTH)(1-44) and anti-hPTH(44-68) antisera and acridinium ester-labeled anti-hPTH(1-34) to simultaneously measure both intact hPTH and its amino-terminal fragments. Results by the assay correlate well with those by a cAMP-based bioassay and the Nichols Allegro immunoradiometric assay. The min...

caviar is one of the valuable and most popular fish products all over the world that are obtained from sturgeons. nowadays, the wild resources of these fishes are diminished, therefore, to meet the demand for the product, farming sturgeons has been considered. the chemical composition and the amino acids profiles of the wild and farmed roe obtained from beluga (huso huso) were compared and the ...

concentration, which is recognized by a sevenIntroduction transmembrane-domain receptor with a large extracellular amino-terminal region, the calcium-sensing The development of secondary hyperparathyroidism receptor [4]. A decrease in extracellular calcium is a major risk factor for the development of renal concentration leads not only to an increase in PTH osteodystrophy. In cases of chronic r...

Early development of the digestive tract is crucial for achieving maximal growth and development of chickens. This study examined the effects of in ovo (IO) feeding of 0.70 mL of dextrose (10.00% and 20.00%) or amino acids solutions into the yolk sac at day 14 of incubation on small intestine histomorphometry and histomorphology, intestinal development, hatchability, body weight, and l...