Non-specific electrostatics is crucial for structure and stability; recently, it has been argued that psychrophilic proteins may also utilize specific electrostatic interactions. Do increase the number of salt bridges cold adaptation? Are there any changes occur in their sequence, which helps them to adapt an extreme environment? intra-protein interactions affect stability? Is special type inte...

During a springtime study of pelagic microbial activities in the coastal Alaskan Arctic (Chukchi Sea, June 1998), a selective serial dilution technique was used to estimate the relative abundance of cold-loving (psychrophilic) and cold-tolerant (psychrotolerant or psychrotrophic) pelagic bacteria at four stations representing different stages of an algal bloom. Psychrophiles were numerically do...

The psychrophilic bacterium Moritella dasanensis strain ArB 0140 was isolated near a glacier in Kongsfjorden, Svalbard Archipelago, Norway. Here we report a 4.89-Mb draft genome sequence of Moritella dasanensis ArB 0140, which could provide comprehensive information on a psychrophilic mechanism in extreme environments.

Larkin, J. M. (Washington State University, Pullman), and J. L. Stokes. Isolation of psychrophilic species of Bacillus. J. Bacteriol. 91:1667-1671. 1966.-Ninety psychrophilic isolates of Bacillus were obtained from soil, mud, and water by selective enrichment at 0 C. They grew well at 0 C, optimally at 20 to 25 C, and failed to grow at 30 or 35 C. Their minimal and maximal growth temperatures w...

Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic enzyme (dihydrofolate reductase) of Moritella profunda sp. nov., a strictly psychrophilic bacterium with a maximal growth rate at 2 degrees C or less. The enzyme is monomeric (Mr=18,291), 55% i...

Shewanella halifaxensis and Shewanella sediminis were among a few aquatic gamma-proteobacteria that were psychrophiles and the first anaerobic bacteria that degraded hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). Although many mesophilic or psychrophilic strains of Shewanella and gamma-proteobacteria were sequenced for their genomes, the genomic evolution pathways for temperature adaptation wer...

Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychr...