Eighty-six Pseudomonas syringae pv. phaseolicola isolates collected from different bean growing areas in Eskişehir were typed for the production of bacteriocin.All the isolates tested produced bacteriocin and 24 bacteriosin groups were determined. No correlation was found between the bacteriocin groups and geographical origin. Authentic isolates of the bacterium representing 3 different races w...

Pseudomonas syringae pv. tomato DC3000 produces seven to eight major extracellular proteins (EXPs) in a minimal medium inducing hrp genes. Using a polyclonal antibody against DC3000 EXPs, we have determined that the production and secretion of five EXPs (EXP-60, EXP-45, EXP-43, EXP-22, and EXP-10) are under the control of the Hrp regulation and secretion system.

Pseudomonas aeruginosa and the phytopathogen P. syringae produce the exopolysaccharide alginate, which is a copolymer of D-mannuronic and L-guluronic acids. One of the key regulatory genes controlling alginate biosynthesis in P. aeruginosa is algT, which encodes the alternate sigma factor, sigma(22). In the present study, the algT gene product from P. syringae pv. syringae showed 90% amino acid...

Pseudomonas syringae pv. syringae, like many plant pathogenic bacteria, secretes a 'harpin' protein that can elicit the hypersensitive response (HR), a defensive cellular suicide, in non-host plants. The harpin-encoding hrpZ gene is located in an operon that also encodes Hrp secretion pathway components and is part of the functional cluster of hrp genes carried on cosmid pHIR11 that enables sap...

Fluorescent Pseudomonas strains producing the antimicrobial secondary metabolite 2,4-diacetylphloroglucinol (Phl) play a prominent role in the biocontrol of plant diseases. A subset of Phl-producing fluorescent Pseudomonas strains, which can additionally synthesize the antimicrobial compound pyoluteorin (Plt), appears to cluster separately from other fluorescent Pseudomonas spp. based on 16S rR...

abstract the ability to monitor particular microorganism in an environment is a difficult task. many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxab)]. therefore, lux-ab gene was cloned into two endemic plant pathogens pseudomonas syringae and ralostonia solanacearum by electrotransformation. l...

Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, and fgt2. In this study, we newly identified vioR and vioM in a so-called ...