Soil microbial diversity is an important index of agricultural productivity by enhancing soil quality. Present study employed the combination of several methodologies to examine genetic relationships among specific groups of bacteria and to characterize strains at the species level using molecular biotyping techniques. The present study is based upon 16SrDNA analysis for characterisation of bac...

The activity of azlocillin and mezlocillin, new semisynthetic ureido penicillins, was investigated and compared with that of other known beta-lactam antibiotics. At a concentration of 25 mug/ml, azlocillin inhibited 74% of Enterobacter, 97% of Proteus mirabilis, 64% of Citrobacter, 91% of Pseudomonas aeruginosa, and 82% of Bacteroides strains tested. Mezlocillin inhibited 86% of Shigella, 96% o...

Two Gram-negative, strictly aerobic bacterial strains, designated CL-AP6(T) and CL-AP22, were isolated from a culture of a green alga, Pyramimonas gelidicola, established from the Antarctic. Cells of the strains were straight rods and motile by means of a single polar flagellum. The strains grew with 0.5-8 % (w/v) NaCl (optimum, 1-2 %) and at 4-33 degrees C (optimum, 25 degrees C) and pH 6.5-9....

The uptake of methylamine as the sole nitrogen, but not carbon, source by Pseudomonas sp. strain MA was investigated. Under these growth conditions, a high-affinity, low-capacity uptake system was present having a Km of 16 microM and Vmax of 2 nmol.min-.mg (dry weight) of cells that was competitively inhibited by ammonium chloride. The transport system was induced by growth on succinate with me...

BACKGROUND Keeping in mind the commercial application of polygalacturonase (PG) in juice and beverages industry, bacterial strains were isolated from rotten fruits and vegetables to screen for competent producers of PG. OBJECTIVES In this study, the plate method was used for preliminary screening of polygalacturonase-producing bacteria, while the Dinitrosalicylic Acid (DNS) method was used fo...

The presence of nitrogen fixers within the genus Pseudomonas has been established and so far most isolated strains are phylogenetically affiliated to Pseudomonas stutzeri. A gene ortholog neighborhood analysis of the nitrogen fixation island (NFI) in four diazotrophic P. stutzeri strains and Pseudomonas azotifigens revealed that all are flanked by genes coding for cobalamin synthase (cobS) and ...

Meloidogyne incognita is a plant pathogen causing root-knot nematodes disease in many crops worldwide. Due to the environmental threat on use of chemical fumigants, there need for biological control method using microbial antagonists disease. Therefore, this study was conducted screen and evaluate biocontrol potential P. fluorescens strains against nematodes. The effectiveness six viz., Pf1, Pf...

Nine laboratory standard strains and 29 clinically isolated strains of Pseudomonas aeruginosa were employed for the studies of combined use of sulbenicillin and gentamicin in vitro. Increased antibacterial activity by the combined use of the two drugs over the activity of either drug alone was observed against all the strains tested. Bactericidal activity against P. aeruginosa N 18 was enhanced...

The response regulator gene gacA influences the production of several secondary metabolites in both pathogenic and beneficial Pseudomonas spp. In this study, we developed primers and a probe for the gacA gene of Pseudomonas species and sequenced a 425 bp fragment of gacA from ten Pseudomonas strains isolated from different plant-associated environments. Polymerase chain reaction analysis and So...

Biodegradation of triazole fungicide propiconazole was carried out in vitro by selected Pseudomonas strains isolated from tea rhizosphere. A total number of twelve strains were isolated and further screened based on their tolerance level to propiconazole. Four best strains were selected and further tested for their nutritional requirements. Among the different carbon sources tested glucose exhi...