Miyawaky et al. have identified recently a new prothrombin gene mutation c.1787G>T in a Japanese family with hereditary thrombophilia [4]. This mutation is located in the last exon of the prothrombin gene resulting in Arg596Leu replacement. Djordjevic et al. [5] also have described another mutation at the same prothrombin gene position (c. 1787G>A prothrombin Belgrade) that results in aminoacid...

A questionnaire indicating the presence of a history or physical findings consistent with liver desease or bleeding disorders was completed by house officers on 301 admissions to a Veterans Administration medical service. Each patient had prothrombin time determined on admission. Only two of 107 patients for whom the prothrombin time was measured as a screening test had an abnormal results and ...

Factor IX Chicago-2 and prothrombin Madrid were purified from patients with hemophilia B and congenital dysprothrombinemia. respectively. Each protein displays defects in zymogen activation secondary to the failure to cleave one of the sessile bonds whose cleavage is necessary for full coagulant activity. These proteins were isolated by immunoaffinity chromatography using conformation-specific ...

The zymogen prothrombin is composed of fragment 1 containing a Gla domain and kringle-1, fragment 2 containing kringle-2, and a protease domain containing A and B chains. The prothrombinase complex assembled on the surface of platelets converts prothrombin to thrombin by cleaving at Arg-271 and Arg-320. The three-dimensional architecture of prothrombin and the molecular basis of its activation ...

Prothrombin has 10 gamma-carboxyglutamic acid residues which are essential for the metal ion binding properties and membrane binding function of the protein. To assess the importance of each gamma-carboxyglutamic acid residue we made, by site directed mutagenesis, a series of mutant human prothrombins each with a single glutamic acid to aspartic acid substitution at positions 6, 7, 14, 16, 19, ...

OBJECTIVE/BACKGROUND Despite being an important risk factor for venous thromboembolism, the role of the prothrombin G20210A mutation in patients with arterial disease remains unclear. The aim of this review was to evaluate the association of prothrombin G20210A and lower extremity peripheral arterial disease (PAD). METHODS This was a systematic review and meta-analysis of case-control studies...

The great advances that have been made in recent years in the study of the coagulation reaction of the blood have come about largely from work done on prothrombin. In order that this progress continue, greater insistence on accuracy in the determination of prothrombin must be made and a more comprehensive view of the nature of prothrombin should be adopted. According to the evidence of the writ...

The great advances that have been made in recent years in the study of the coagulation reaction of the blood have come about largely from work done on prothrombin. In order that this progress continue, greater insistence on accuracy in the determination of prothrombin must be made and a more comprehensive view of the nature of prothrombin should be adopted. According to the evidence of the writ...