introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

Protein and DNA have vital role in our biological processes. For accurately predicting binding protein, develop a new sequence based prediction method from the protein sequence. Sequence only considers information as input. DBP, first reliable benchmark data set bank. Second, using Amino Acid Composition (AAC), Position Specific Scoring Matrix (PSSM), Predicted Solvent Accessibility (PSA), Prob...

background: nontypeable haemophilus influenzae (nthi) is a common cause of respiratory tract disease and initiates infection by colonization in nasopharynx. the haemophilus influenzae (h. influenzae) hap adhesin is an auto transporter protein that promotes initial interaction with human epithelial cells. hap protein contains a 110 kda internal passenger domain called “haps” and a 45 kda c-termi...