Lysozyme, as a model protein, was precipitated through the formation of protein-Zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (PLGA) microspheres. Various parameters, including pH, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. The resulting protein parti...

prosite database contains a set of entries corresponding to protein families, which are used to identify the family of a protein from its sequence. although patterns and profiles are developed to be very selective, each may have false positive or negative hits. considering false positives as items that reduce the selectiveness of a pattern, then, the more selective pattern we have, a more accur...

The particle size of wheat bran plays an important role in the quality reconstituted whole-wheat flour and its products. effects on cooked noodles were analyzed; mean (D50) ranged from 26.05 to 46.08 μm. Results show that decreases D50 induced changes noodles. Specifically, damaged starch content, water absorption, solvent retention capacity sodium carbonate sucrose increased at various degrees...

Background and objectives Durum wheat (Triticum durum Desf.), such as other cereals, contains several interesting bioactive compounds mostly found within the coating structure of kernel; also, these structures included in bran fractions, contain highest concentration both minerals organic contaminants (mycotoxins). The purpose this project was to employ micronization air-classification technolo...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...