A cDNA coding for 3-ketoacyl-acyl-carrier protein (ACP) synthase III (KAS III) from spinach (Spinacia oleracea; So KAS III) was used to isolate two closely related KAS III clones (Ch KAS III-1 and Ch KAS III-2) from Cuphea hookeriana. Both Ch KAS IIIs are expressed constitutively in all tissues examined. An increase in the levels of 16:0 was observed in tobacco (Nicotiana tabacum, WT-SR) leaves...

Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to drought stress were studied in three rapeseed cultivars. For the cultivar 2AF009 with high drought tolerance, comparative proteomic analyses were conducted to determine the molecular mechanism behind. Approximately 138 differentially abu...

The effect of aqueous smoke extract, derived from Tanacetum parthenium, onin vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)microspore-derived embryos (MDEs) was evaluated in this study. Inoculation ofrapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)for various times (5, 10 and 15 min) significantly enhanced the percentage ofregenerated pla...

Lysophosphatidic acid acyltransferase acylates the sn-2 hydroxyl group of lysophosphatidic acid to form phosphatidic acid, a precursor to triacylglycerol. A cDNA encoding lysophosphatidic acid acyltransferase was isolated from developing seeds of meadowfoam (Limnanthes alba alba). The cDNA encodes a 281-amino acid protein with a molecular mass of 32 kD. The cDNA was expressed in developing seed...

272 India accounts for 12-15% of world’s oilseed area, 7-8% of oilseeds output, 6-7% of vegetable oil production, 9-12% of vegetable oil import and 9-10% of vegetable oil consumption (Hegde, 2009). The country produces seven edible oilseed crops viz. groundnut, rapeseed-mustard, soybean, sunflower, sesame, safflower and niger and two non edible oilseeds viz. castor and linseed. Though the diver...

Rapeseed has formed three ecological types, namely winter, semi-winter, and spring during the long domestication process. Different ecotypes have different low-temperature requirements for floral initiation. growth a large demand nitrogen, amount of nitrogen application significantly impacts number flowering. Meanwhile, time imitation determined quantity bud, final pods, yield rapeseed. Therefo...

The use of transgenic crops as feeds for ruminant animals has prompted study of the possible uptake of transgene fragments by ruminal micro-organisms and/or intestinal absorption of fragments surviving passage through the rumen. The persistence in buffered ruminal contents of seven different recombinant DNA fragments from GM rapeseed expressing the 5-enolpyruvylshikimate-3-phosphate synthase (E...

BACKGROUND Single nucleotide polymorphisms (SNPs) are an important class of genetic marker for target gene mapping. As of yet, there is no rapid and effective method to identify SNPs linked with agronomic traits in rapeseed and other crop species. METHODOLOGY/PRINCIPAL FINDINGS We demonstrate a novel method for identifying SNP markers in rapeseed by deep sequencing a representative library an...

Fruit and seed development in plants is a complex biological process mainly involved in input and biosynthesis of many storage compounds such as proteins and oils. Although the basic biochemical pathways for production of the storage metabolites in plants are well characterized, their regulatory mechanisms are not fully understood. In this study, we functionally identified rapeseed (Brassica na...

26 This narrative review compares evidence from experimental, epidemiological and 27 clinical studies of the health benefits of rapeseed (Canola) oil and olive oil in order to 28 assess if rapeseed oil is suitable as a sustainable alternative to olive oil as part of a 29 Mediterranean-style diet in countries where olive trees do not grow. From 30 epidemiological studies, the evidence for cardio...