OBJECTIVE To investigate the association between smoking, semen quality, and the histone-to-protamine transition ratio in mature sperm. DESIGN Biochemical and molecular analysis in human samples and a cell line. SETTING Andrology laboratory in a university-affiliated hospital. PATIENT(S) Semen samples from 147 heavy smokers and 175 nonsmokers receiving infertility treatment. INTERVENTIO...

Cardiac surgical case histories, collected over 4 years at Huntsville Hospital in Alabama, were reviewed for 2,293 patients. Patients were separated into two dosing groups for both heparin and protamine, hospital empirically dosed and Hemochron RxDx dosed. Review of the heparin dosing information found that incomplete data were collected for 47 patients, leaving 2,246 patients eligible to be ev...

OBJECTIVE We investigated the mechanism of the protamine action and the effects of free hemoglobin on protamine-induced responses in endothelium-denuded and-intact human internal thoracic artery (ITA) rings precontracted with phenylephrine (PE) or high KCl. METHODS Samples of redundant ITA obtained from patients undergoing a coronary artery bypass graft surgery were cut into 3 mm wide rings a...

Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims evaluate characteristics semen Limousin bulls different ages, which still used for programs up 12 years age. ages 3, 8, produced in 2021 at Artificial Insemination Center Singosari, East Java Province, ...

OBJECTIVE To determine whether the incidence of protamine-induced pulmonary vasoconstriction (PIPV) is influenced by central venous versus peripheral venous infusion of protamine and whether aspirin ingestion within a week of surgery would decrease the incidence of PIPV. DESIGN Single-institution, prospective, observational, randomized trial. SETTING University teaching hospital. PARTICIP...

Protamine, a small, strongly positively-charged protein, plays key role in achieving chromatin condensation inside sperm cells and is also involved the formulation of nanoparticles for gene therapy and...

Protamine mRNA was isolated in a very pure form from trout testis and used as a template for the synthesis of a complementary DNA of high specific activity. The cDNA represented a full copy of the mRNA template and was used in hybridization reactions with purified trout DNA to determine the number of genes for protamines in trout testis. Our results indicate that there are less than four genes ...

Cysteine oxidation in protamines leads to their oligomerization and contributes to sperm chromatin compaction. Here we identify the Drosophila thioredoxin Deadhead (DHD) as the factor responsible for the reduction of intermolecular disulfide bonds in protamines and their eviction from sperm during fertilization. Protamine chaperone TAP/p32 dissociates DNA-protamine complexes in vitro only when ...

Here, we report the case of a patient who developed protamine shock during a transcatheter aortic valve implant (TAVI) procedure, which was diagnosed by intraoperative transesophageal echocardiography (TEE). A 77-year-old man with symptomatic severe aortic stenosis and reduced left ventricular (LV) function underwent TAVI under general anesthesia. During the procedure, a transcatheter heart val...