نتایج جستجو برای: pronuclear formation

تعداد نتایج: 527908  

Journal: :Reproductive BioMedicine Online 2008

2017

Although, the first transgenic animal was produced more than 30 years ago, there are still many factors limiting efficiency of transgenesis. One of them is the low rate of transgene incorporation into the genome of microinjected eggs. This mini-review summarizes recent research methods based on pronuclear microinjection of rabbit fertilized eggs in order to reach higher efficiency of transgene ...

Journal: :The Journal of Cell Biology 1982
D P Kiehart I Mabuchi S Inoué

Antibody against cytoplasmic myosin, when microinjected into actively dividing cells, provides a physiological test for the role of actin and myosin in chromosome movement. Anti-Asterias egg myosin, characterized by Mabuchi and Okuno (1977, J. Cell Biol., 74:251), completely and specifically inhibits the actin activated Mg++ -ATPase of myosin in vitro and, when microinjected, inhibits cytokines...

Journal: :Human reproduction 2008
A Beuchat P Thévenaz M Unser T Ebner A Senn F Urner M Germond C O S Sorzano

BACKGROUND Identification of embryos with high implantation potential remains a challenge in in vitro fertilization (IVF). Subjective pronuclear (PN) zygote scoring systems have been developed for that purpose. The aim of this work was to provide a software tool that enables objective measuring of morphological characteristics of the human PN zygote. METHODS A computer program was created to ...

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