Embryogenic callus was induced from culturing immature embryos of three barley cvs. Dissa, Golden Promise and Igri. Maximum embryogenic callus formation was achieved by culturing immature embryos on CC and LS medium containing 2 mg/litre and 2.5 mg/litre 2,4dichlorophenoxyacetic acid (2,4-D) respectively. Embryogenic calli were frequently produced distinguished somatic embryos on these medium. ...

Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...

On-orbit servicing (OOS) presents new opportunities for refueling, inspection, repair, maintenance, and upgrade of spacecraft (s/c). OOS missions s/c in a geostationary orbit (GEO) are currently underway. However, there no plans low-Earth-orbit (LEO) s/c, aside from technology demonstrations, because their shorter design life lower cost. Designing systems LEO constellations differs that GEO-bas...

Global warming is one of the most serious problems leading to changes in distribution areas species and biodiversity. Rhodiola quadrifida a rare plant with adaptogenic properties grows highlands narrow temperature range 2–15 °C. The aim our work was study growth content main metabolites two vitro cultures at temperatures 5, 15 25 Hairy roots calli were cultivated on agar medium for 28 days. max...

In the present study, we first attempted to achieve an efficient procedure for optimizing callogenesis from apical meristem and leaf explants of Salvia tebesana on MS media containing different concentrations BAP alone in combination with 2,4-D. Then, inducing effect nano-TiO2 (10, 60, 120 mg L−1) methyl jasmonate (50, 100, 200 µM), as abiotic elicitors were studied enhancement phenolic compoun...

Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

Abstract In vitro-derived plants with elevated ploidy levels can display distinguishing features from the they are derived from, especially owing to their indirect regeneration. Genome size affects cell and, thus, plant morphological characteristics. Therefore, stomata traits may be used as a diagnostic feature allowing identification of ploidy. Actinidia chinensis var. deliciosa A. Chev. (A. C...

St. Augustinegrass is a widely used turf and pasture grass in the southern US. Raleigh is a cultivar known for superior cold tolerance than other St. Augustinegrass cultivars. However, its coarse-leaf texture and long internodes are undesirable when planted in home lawns. Mutagenesis by gamma ray irradiation was employed to treat node cuttings and calli for inducing semi-dwarf growth phenotype....