BACKGROUND Expression of recombinant antibodies and their derivatives fused with other functional molecules such as alkaline phosphatase in Escherichia coli is important in the development of molecular diagnostic reagents for biomedical research. METHODS We investigated the possibility of applying a well-known Fos-Jun zipper to dimerize V(H) and V(L) fragments originated from the Fab clone (S...

in the present study, randomly amplified polymorphic dna (rapd) markers were used to analyze the genetic diversity in 20 varieties of philodendron. the polymerase chain reaction was performed with 60 rapd primers, out of which 21 primers showed clear amplification as well as more polymorphism. in total, 354 scorable rapd loci with 348 polymorphic bands (98%) were observed. percentages of polymo...

A method is described which utilizes pulse labeling with Sa5, starch gel electrophoresis, and autoradiography to detect the synthesis of proteins at various times after infection of Salmonella typhimurium with phage P22. The system was developed especially to study proteins involved in lysogeny. A comparison was made of various infections. These involve the wild-type phage (c+) under conditions...

A procedure for mapping small DNA probes directly on banded human chromosomes by fluorescence in situ hybridization has been developed. This procedure allows for the simultaneous visualization of banded chromosomes and hybridization signal without overlaying two separate photographic images. This method is simple and rapid, requires only a typical fluorescence microscope, has proven successful ...

We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fi...

The impact of ultra violet radiations on alpha amylase producing ability of Bacillus subtilis (2620) was studied. Maximum enzymatic activity (crude) upto 17 folds were obtained from mutant strain exposed to 60 minutes ultra violet irradiation in comparison to parent strain. Purified enzyme showed molecular weight of 68 KDa and 54 KDa by SDS-PAGE in both parent and mutant strains. The SDS-PAGE o...

Twelve Aspergillus sp. strains producing glucose dehydrogenase were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was investigated. Moreover, partial gdh gene sequences were determined and aligned. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of twelve Aspergillus isola...

An activity gel assay was developed for the detection of DNA helicases in crude extracts. The assay was based on the ability of DNA helicases to unwind radioactive fragments from single-stranded M13 circles that were immobilized in an SDS polyacrylamide gel. The displaced radioactive strands were detected by blotting them to a filter and visualizing the resulting bands by autoradiography. Exper...

An Egyptian female with night sweats, headache, and back pain was diagnosed with acute brucellosis one week after returning from a North African country. Humoral immune responses to specific immunogenic proteins were investigated before and after treatment. ELISA was performed to detect levels of specific antibody (Ab) titers. Immunoblot analysis of Ab recognizing specific Brucella antigenic ba...

The advent of DNA technology over the recent years has led to the development of molecular markers that are highly precise, convenient and cost effective for detection of polymorphism among individuals and also for individual identification. These molecular marker techniques viz., Restriction fragment length polymorphism, Random amplified polymorphic DNA, Amplified fragment length polymorphism,...